Skip to main content
SpringerLink
Log in

Platelets and Megakaryocytes

Volume 3, Additional Protocols and Perspectives

  • Book
  • © 2012

Overview

Editors:
  1. Jonathan M. Gibbins

    1. School of Biological Sciences, Inst. Cardiovascular & Metabolic Res., University of Reading, Reading, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Martyn P. Mahaut-Smith

    1. , Dept. Cell Physiology & Pharmacology, University of Leicester, Leicester, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Compliments the two previous volumes by supplying fully up-to-date techniques
  • Includes many detailed protocols that are lab ready and tested
  • Features expert tips and vital implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 788)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Additional Protocols for the Study of Platelet Function

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally Induced Vascular Damage and Plaque Rupture In Vivo

      • Marijke J. E. Kuijpers, Johan W. M. Heemskerk
      Pages 3-19

    3. Assessment of Platelet Aggregation Responses In Vivo in the Mouse

      • Christopher Moore, Michael Emerson
      Pages 21-28

    4. Focal Cerebral Ischemia

      • Stefan Braeuninger, Christoph Kleinschnitz, Bernhard Nieswandt, Guido Stoll
      Pages 29-42

    5. Extracorporeal Assays of Thrombosis

      • Lina Badimon, Teresa Padro, Gemma Vilahur
      Pages 43-57

    6. Platelet Life Span and Apoptosis

      • Emma C. Josefsson, Michael J. White, Mark R. Dowling, Benjamin T. Kile
      Pages 59-71

    7. A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow

      • Warwick S. Nesbitt, Ian S. Harper, Simone M. Schoenwaelder, Yuping Yuan, Shaun P. Jackson
      Pages 73-89

    8. Platelet Shape Change and Spreading

      • Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, Owen J. T. McCarty
      Pages 91-100

    9. Clot Retraction

      • Katherine L. Tucker, Tanya Sage, Jonathan M. Gibbins
      Pages 101-107

    10. Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton

      • Jonathan N. Thon, Joseph E. Italiano
      Pages 109-125

    11. Measurement of Platelet Microparticles

      • Jeffrey I. Zwicker, Romaric Lacroix, Françoise Dignat-George, Barbara C. Furie, Bruce Furie
      Pages 127-139

    12. Assessing Protein Synthesis by Platelets

      • Hansjörg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, Andrew S. Weyrich
      Pages 141-153

    13. A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies

      • Stefan Amisten
      Pages 155-172

  3. Additional Protocols for the Study of Megakaryocyte Function

    1. Front Matter

      Pages 173-173
      Download chapter PDF

    2. Characterization of Megakaryocyte Development in the Native Bone Marrow Environment

      • Anita Eckly, Catherine Strassel, Jean-Pierre Cazenave, François Lanza, Catherine Léon, Christian Gachet
      Pages 175-192

    3. Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow

      • Harald Schulze
      Pages 193-203

    4. In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

      • Naoya Takayama, Koji Eto
      Pages 205-217

    5. Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells

      • Amélie Robert, Valérie Cortin, Alain Garnier, Nicolas Pineault
      Pages 219-247

    6. Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line

      • Yumiko Matsubara, Mitsuru Murata, Yasuo Ikeda
      Pages 249-258
Back to top

Keywords

About this book

New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two volumes published in 2004 by adding recently developed state of the art techniques.  Conveniently divided into three sections, this detailed volume covers techniques to study platelet function, approaches to investigate megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.

Editors and Affiliations

  • School of Biological Sciences, Inst. Cardiovascular & Metabolic Res., University of Reading, Reading, United Kingdom

    Jonathan M. Gibbins

  • , Dept. Cell Physiology & Pharmacology, University of Leicester, Leicester, United Kingdom

    Martyn P. Mahaut-Smith

Bibliographic Information

  • Book Title: Platelets and Megakaryocytes

  • Book Subtitle: Volume 3, Additional Protocols and Perspectives

  • Editors: Jonathan M. Gibbins, Martyn P. Mahaut-Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-307-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-306-6Published: 02 December 2011

  • Softcover ISBN: 978-1-4939-5847-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-307-3Published: 02 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 409

  • Topics: Cell Biology, Cell Culture

Publish with us

Policies and ethics

Back to top

Search

Navigation