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Protein Microarrays

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Ulrike Korf

    1. (DKFZ), Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany

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  • Collects key protein microarray strategies and summarizes the most up-to-date advances
  • Features step-by-step, lab-ready protocols
  • Includes expert tips and vital implementation advice to assure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 785)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Reverse Phase Protein Arrays

    1. Front Matter

      Pages 1-1
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    2. Reverse Phase Protein Microarrays for Clinical Applications

      • Mariaelena Pierobon, Claudio Belluco, Lance A. Liotta, Emanuel F. Petricoin III
      Pages 3-12

    3. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays

      • Kristi Ambroz
      Pages 13-21

    4. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology

      • Virginia Espina, Claudius Mueller, Lance A. Liotta
      Pages 23-43

    5. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays

      • Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, Özgür Sahin
      Pages 45-54

    6. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification

      • Jan C. Brase, Heiko Mannsperger, Holger Sültmann, Ulrike Korf
      Pages 55-64

    7. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models

      • Satoshi S. Nishizuka
      Pages 65-77

    8. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays

      • Georg Martiny-Baron, Dorothea Haasen, Daniel D’Dorazio, Johannes Voshol, Doriano Fabbro
      Pages 79-107

    9. Use of Formalin-Fixed and Paraffin-Embedded Tissues for Diagnosis and Therapy in Routine Clinical Settings

      • Daniela Berg, Katharina Malinowsky, Bilge Reischauer, Claudia Wolff, Karl-Friedrich Becker
      Pages 109-122

    10. Producing Reverse Phase Protein Microarrays from Formalin-Fixed Tissues

      • Claudia Wolff, Christina Schott, Katharina Malinowsky, Daniela Berg, Karl-Friedrich Becker
      Pages 123-140

    11. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned

      • Steven M. Kornblau, Kevin R. Coombes
      Pages 141-155

  3. Antibody Microarrays

    1. Front Matter

      Pages 157-157
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    2. Antibody Microarrays as Tools for Biomarker Discovery

      • Marta Sanchez-Carbayo
      Pages 159-182

    3. Assessment of Antibody Specificity Using Suspension Bead Arrays

      • Jochen M. Schwenk, Peter Nilsson
      Pages 183-189

    4. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays

      • Frauke Henjes, Frank Götschel, Anika Jöcker, Ulrike Korf
      Pages 191-201

    5. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Colour Mode

      • Christoph Schröder, Mohamed S. S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, Jörg D. Hoheisel
      Pages 203-221

    6. High-Throughput Studies of Protein Glycoforms Using Antibody–Lectin Sandwich Arrays

      • Brian B. Haab, Tingting Yue
      Pages 223-236

    7. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies

      • Johanna Sonntag, Heiko Mannsperger, Anika Jöcker, Ulrike Korf
      Pages 237-245

    8. Recombinant Antibodies for the Generation of Antibody Arrays

      • Carl A. K. Borrebaeck, Christer Wingren
      Pages 247-262
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Keywords

About this book

Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable.  In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Protein Microarrays: Methods and Protocols aims to stimulate the application and further advancement of this powerful technology in labs worldwide.

Editors and Affiliations

  • (DKFZ), Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Ulrike Korf

Bibliographic Information

  • Book Title: Protein Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Ulrike Korf

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-286-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-285-4Published: 19 August 2011

  • Softcover ISBN: 978-1-4939-5776-7Published: 23 August 2016

  • eBook ISBN: 978-1-61779-286-1Published: 08 September 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 398

  • Topics: Protein Science, Microarrays

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