Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 764)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.
Reviews
From the reviews:
“The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. … This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one.” (Omer Iqbal, Doody’s Book Reviews, November, 2011)Editors and Affiliations
Bibliographic Information
Book Title: Therapeutic Oligonucleotides
Book Subtitle: Methods and Protocols
Editors: John Goodchild
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-188-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-187-1Published: 15 July 2011
Softcover ISBN: 978-1-4939-5822-1Published: 23 August 2016
eBook ISBN: 978-1-61779-188-8Published: 15 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 340
Number of Illustrations: 70 b/w illustrations