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Signal Transduction Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Louis M. Luttrell

    1. Div. Endocrinology, Metabolism &, Medical Genetics, Medical University of South Carolina, Charleston, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Stephen S. G. Ferguson

    1. Dept. Physiology & Pharmacology, University of Western Ontario, London, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 756)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overviews

    1. Front Matter

      Pages 1-1
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    2. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling

      • Louis M. Luttrell, Terry P. Kenakin
      Pages 3-35

    3. Imaging-Based Approaches to Understanding G Protein-Coupled Receptor Signalling Complexes

      • Darlaine Pétrin, Terence E. Hébert
      Pages 37-60

    4. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis

      • John K. Westwick, Jane E. Lamerdin
      Pages 61-73

    5. RGS-Insensitive Gα Subunits: Probes of Gα Subtype-Selective Signaling and Physiological Functions of RGS Proteins

      • Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, Richard R. Neubig
      Pages 75-98

    6. Bioinformatic Approaches to Metabolic Pathways Analysis

      • Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, Sung-Soo Park
      Pages 99-130

  3. Receptor–Ligand Interactions

    1. Front Matter

      Pages 131-131
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    2. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET

      • Jean-Pierre Vilardaga
      Pages 133-148

    3. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signalling Complexes

      • Nicolas Audet, Graciela Piñeyro
      Pages 149-163

  4. Receptor–Receptor Interactions

    1. Front Matter

      Pages 165-165
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    2. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Density Lipoprotein Particles

      • Gisselle A. Vélez-Ruiz, Roger K. Sunahara
      Pages 167-182

    3. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Heterodimerization

      • Lamia Achour, Maud Kamal, Ralf Jockers, Stefano Marullo
      Pages 183-200

    4. Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

      • Laëtitia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Eric Trinquet, Laurent Prézeau
      Pages 201-214

    5. Analysis of GPCR/Ion Channel Interactions

      • Christophe Altier, Gerald W. Zamponi
      Pages 215-225

  5. Receptor–Effector Coupling

    1. Front Matter

      Pages 227-227
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    2. Multicolor BiFC Analysis of G Protein βγ Complex Formation and Localization

      • Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, Catherine H. Berlot
      Pages 229-243

    3. Real-Time BRET Assays to Measure G Protein/Effector Interactions

      • Darlaine Pétrin, Mélanie Robitaille, Terence E. Hébert
      Pages 245-261

    4. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

      • Brock F. Binkowski, Frank Fan, Keith V. Wood
      Pages 263-271

    5. Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters

      • Lianne B. Dale, Stephen S. G. Ferguson
      Pages 273-281
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Keywords

About this book

It is increasingly clear that signal transduction is a highly organized and integrated process.  Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction.  Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions.  The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.

Reviews

From the reviews of the third edition:

“This well-illustrated book on signal transduction protocols is a valuable resource for scientists interested in learning the intricate details of complex signaling events that take place within the cell … . Although written for the scientists in the field of signal transduction research, it also will be useful for graduate students and other investigators in pharmacology interested in this area. … Given the rapid advances in the field of signal transduction, this third edition is essential.” (Omer Iqbal, Doody’s Book Reviews, March, 2012)

Editors and Affiliations

  • Div. Endocrinology, Metabolism &, Medical Genetics, Medical University of South Carolina, Charleston, USA

    Louis M. Luttrell

  • Dept. Physiology & Pharmacology, University of Western Ontario, London, Canada

    Stephen S. G. Ferguson

Bibliographic Information

  • Book Title: Signal Transduction Protocols

  • Editors: Louis M. Luttrell, Stephen S. G. Ferguson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-160-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-159-8Published: 30 August 2011

  • Softcover ISBN: 978-1-4939-6108-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-160-4Published: 27 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 430

  • Topics: Cell Biology, Protein-Ligand Interactions

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