Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 745)
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Table of contents (32 protocols)
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Front Matter
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Genetic and Molecular Biological Approaches with Yeast
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Front Matter
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Genetic and Molecular Biological Approaches with Higher Eukaryotes
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Front Matter
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Keywords
About this book
Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube. Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.
Editors and Affiliations
Bibliographic Information
Book Title: DNA Recombination
Book Subtitle: Methods and Protocols
Editors: Hideo Tsubouchi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-129-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-128-4Published: 09 June 2011
Softcover ISBN: 978-1-4939-5778-1Published: 23 August 2016
eBook ISBN: 978-1-61779-129-1Published: 10 June 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 565