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Serum/Plasma Proteomics

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Richard J. Simpson

    1. Ludwig Inst. for Cancer Research, Joint Proteomics Laboratory (JPSL), Royal Melbourne Hospital, Parkville, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. David W. Greening

    1. Ludwig Inst. for Cancer Research, Joint Proteomics Laboratory, Royal Melbourne Hospital, Parkville, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 728)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Fractionation Strategies for In-depth Blood Proteome Analysis

    1. Front Matter

      Pages 1-1
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    2. Plasma Biomarker Discovery Using 3D Protein Profiling Coupled with Label-Free Quantitation

      • Lynn A. Beer, Hsin-Yao Tang, Kurt T. Barnhart, David W. Speicher
      Pages 3-27

    3. Intact Protein Separation by One- and Two-Dimensional Liquid Chromatography for the Comparative Proteomic Separation of Partitioned Serum or Plasma

      • Simon Sheng, Helena Skalnikova, Andrew Meng, John Tra, Qin Fu, Allen Everett et al.
      Pages 29-46

    4. In-Depth Analysis of a Plasma or Serum Proteome Using a 4D Protein Profiling Method

      • Hsin-Yao Tang, Lynn A. Beer, David W. Speicher
      Pages 47-67

    5. Intact-Protein Analysis System for Discovery of Serum-Based Disease Biomarkers

      • Hong Wang, Samir Hanash
      Pages 69-85

    6. Model-Based Discovery of Circulating Biomarkers

      • Maryann S. Vogelsang, Kian Kani, Jonathan E. Katz, Parag Mallick
      Pages 87-107

    7. Low-Molecular Weight Plasma Proteome Analysis Using Centrifugal Ultrafiltration

      • David W. Greening, Richard J. Simpson
      Pages 109-124

    8. High-Throughput Analysis of Glycoproteins from Plasma

      • Yan Li, Hui Zhang
      Pages 125-133

  3. Blood Collection and Handling Strategies

    1. Front Matter

      Pages 135-135
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    2. Minimizing Preanalytical Variation of Plasma Samples by Proper Blood Collection and Handling

      • Jizu Yi, David Craft, Craig A. Gelfand
      Pages 137-149

    3. Collection and Handling of Blood Specimens for Peptidomics

      • Harald Tammen, Rüdiger Hess
      Pages 151-159

    4. Investigation of Peptide Biomarker Stability in Plasma Samples Using Time-Course MS Analysis

      • Jizu Yi, Zhaoxia Liu, Craig A. Gelfand, David Craft
      Pages 161-175

  4. Methods for Quantitative Assaying of Blood-Based Biomarkers

    1. Front Matter

      Pages 177-177
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    2. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-Glycosites

      • Reto Ossola, Ralph Schiess, Paola Picotti, Oliver Rinner, Lukas Reiter, Ruedi Aebersold
      Pages 179-194

    3. A Fluorescent Microsphere-Based Method for Assay of Multiple Analytes in Plasma

      • Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, Richard J. Simpson
      Pages 195-206

    4. Immuno-Mass Spectrometry: Quantification of Low-Abundance Proteins in Biological Fluids

      • Vathany Kulasingam, Christopher R. Smith, Ihor Batruch, Eleftherios P. Diamandis
      Pages 207-218

    5. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-Reducing Sugars

      • Feliciano Priego-Capote, Maria Ramírez-Boo, Denis Hochstrasser, Jean-Charles Sanchez
      Pages 219-232

  5. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-Related Fluids

    1. Front Matter

      Pages 233-233
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  6. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids

    1. Exosome Isolation for Proteomic Analyses and RNA Profiling

      • Douglas D. Taylor, Wolfgang Zacharias, Cicek Gercel-Taylor
      Pages 235-246
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Keywords

About this book

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring.  Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome.  These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to thefield in an effort to further our knowledge of this fundamental science.

Editors and Affiliations

  • Ludwig Inst. for Cancer Research, Joint Proteomics Laboratory (JPSL), Royal Melbourne Hospital, Parkville, Australia

    Richard J. Simpson

  • Ludwig Inst. for Cancer Research, Joint Proteomics Laboratory, Royal Melbourne Hospital, Parkville, Australia

    David W. Greening

Bibliographic Information

  • Book Title: Serum/Plasma Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Richard J. Simpson, David W. Greening

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-068-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-067-6Published: 12 April 2011

  • Softcover ISBN: 978-1-4939-5801-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-068-3Published: 06 April 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 397

  • Topics: Protein Science, Proteomics

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