Editors:
- Features methods and protocols relevant for both fixed and live cells from everything from bacteria to mammalian models
- Provides detailed techniques from established and up-and-coming experts in the field
- Includes vital notes on troubleshooting and avoiding known pitfalls for easier implementation of the methods
Part of the book series: Methods in Molecular Biology (MIMB, volume 714)
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Table of contents (28 protocols)
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Front Matter
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Visualizing mRNAs In Situ
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Front Matter
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Visualizing mRNAs In Vivo Using Molecular Probes or Reconstituted Fluorescent Proteins
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Front Matter
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Visualizing mRNAs In Vivo Using Aptamers and Intact Fluorescent Proteins
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Front Matter
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About this book
With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.Editors and Affiliations
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, Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
Jeffrey E. Gerst
Bibliographic Information
Book Title: RNA Detection and Visualization
Book Subtitle: Methods and Protocols
Editors: Jeffrey E. Gerst
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-005-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-004-1Published: 06 April 2011
Softcover ISBN: 978-1-4939-5836-8Published: 23 August 2016
eBook ISBN: 978-1-61779-005-8Published: 23 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 497
Topics: Cell Biology, Nucleic Acid Chemistry