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Metabolic Profiling

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Thomas O. Metz

    1. Fundamental & Computational Sciences Dir, Pacific Northwest National Laboratory, Richland, USA

    View editor publications

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  • Illustrates the evolution of metabolic profiling from single molecule profiling to global metabolome profiling
  • Features cutting-edge protocols, presented from the perspective of leading experts in the field
  • Includes valuable notes highlighting tips on troubleshooting and avoiding known methodological pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 708)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Origins of Metabolic Profiling

    • Arthur B. Robinson, Noah E. Robinson
    Pages 1-23

  3. Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism

    • Monique Piraud, Séverine Ruet, Sylvie Boyer, Cécile Acquaviva, Pascale Clerc-Renaud, David Cheillan et al.
    Pages 25-53

  4. Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry

    • David S. Millington, Robert D. Stevens
    Pages 55-72

  5. Analysis of Organic Acids and Acylglycines for the Diagnosis of Related Inborn Errors of Metabolism by GC- and HPLC-MS

    • Giancarlo la Marca, Cristiano Rizzo
    Pages 73-98

  6. HPLC Analysis for the Clinical–Biochemical Diagnosis of Inborn Errors of Metabolism of Purines and Pyrimidines

    • Giuseppe Lazzarino, Angela Maria Amorini, Valentina Di Pietro, Barbara Tavazzi
    Pages 99-117

  7. Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (UPLC/ESI-MS)

    • Masahito Hagio, Megumi Matsumoto, Satoshi Ishizuka
    Pages 119-129

  8. Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry

    • Jan C. van Dam, Cor Ras, Angela ten Pierick
    Pages 131-146

  9. Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry

    • Rajan S. Kombu, Henri Brunengraber, Michelle A. Puchowicz
    Pages 147-157

  10. Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS)

    • Amber Jannasch, Miroslav Sedlak, Jiri Adamec
    Pages 159-171

  11. High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling

    • Ian D. Wilson
    Pages 173-190

  12. Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics

    • Antonia Garcia, Coral Barbas
    Pages 191-204

  13. The Use of Two-Dimensional Gas Chromatography–Time-of-Flight Mass Spectrometry (GC×GC–TOF-MS) for Metabolomic Analysis of Polar Metabolites

    • Kimberly Ralston-Hooper, Amber Jannasch, Jiri Adamec, Maria Sepúlveda
    Pages 205-211

  14. LC-MS-Based Metabolomics

    • Sunil Bajad, Vladimir Shulaev
    Pages 213-228

  15. Capillary Electrophoresis–Electrospray Ionization-Mass Spectrometry (CE–ESI-MS)-Based Metabolomics

    • Philip Britz-McKibbin
    Pages 229-246

  16. Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues

    • Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Orešič
    Pages 247-257

  17. Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-Based Shotgun Lipidomics

    • Giorgis Isaac
    Pages 259-275

  18. Processing and Analysis of GC/LC-MS-Based Metabolomics Data

    • Elizabeth Want, Perrine Masson
    Pages 277-298

  19. Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling

    • Eva M. Lenz
    Pages 299-319

  20. Nuclear Magnetic Resonance (NMR)-Based Metabolomics

    • Hector C. Keun, Toby J. Athersuch
    Pages 321-334
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Keywords

About this book

At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

Reviews

From the reviews:

“This is a recipe book suitable for graduate students and research scientists who are working on metabolic profiling experiments in universities, research institutes and private companies. This book addresses the targeted profiling of subfamilies of endogenous metabolites and non-targeted metabolomics performed on various analytical platforms. This book, written systematically by expert scientists in the respective fields, is a valuable resource for scientists working on metabolic profiling.” (Eric Chun Yong Chan, Analytical and Bioanalytical Chemistry, Vol. 403, 2012)

“This is an excellent resource for protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling … . The book is intended for both established and new investigators in the field of metabolic profiling … . an excellent book on the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. In addition to investigators in this field, interested clinicians and endocrinologists will find this book extremely helpful.” (Omer Iqbal, Doody’s Book Reviews, June, 2011)

Editors and Affiliations

  • Fundamental & Computational Sciences Dir, Pacific Northwest National Laboratory, Richland, USA

    Thomas O. Metz

Bibliographic Information

  • Book Title: Metabolic Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas O. Metz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61737-985-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61737-984-0Published: 11 January 2011

  • Softcover ISBN: 978-1-4939-5816-0Published: 23 August 2016

  • eBook ISBN: 978-1-61737-985-7Published: 05 January 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 391

  • Topics: Biochemistry, general, Metabolomics

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