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Adipose-Derived Stem Cells

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Jeffrey M. Gimble

    1. , Stem Cell Biology, Pennington Biomedical Research Center, Baton Rouge, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bruce A. Bunnell

    1. School of Medicine, Department of Pharmacology, Tulane University, New Orleans, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Focuses on human adipose-derived stem cells with additional coverage of ASCs of small and large animal species
  • Features detailed protocols accessible to new students and indispensible to experienced researchers
  • Includes tips from the expert contributors on troubleshooting and implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 702)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
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  2. Isolation Methods

    1. Front Matter

      Pages 1-1
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    2. Isolation and Culture of Rhesus Adipose-Derived Stem Cells

      • Christine Gagliardi, Bruce A. Bunnell
      Pages 3-16

    3. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

      • Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, Jeffrey M. Gimble
      Pages 17-27

    4. Isolation of Murine Adipose-Derived Stem Cells

      • Gang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M. Gimble, Z. Elizabeth Floyd
      Pages 29-36

    5. In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation

      • Mandi J. Lopez, Nakia D. Spencer
      Pages 37-46

    6. In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics

      • Nakia D. Spencer, Mandi J. Lopez
      Pages 47-60

    7. Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells

      • Martin A. Vidal, Mandi J. Lopez
      Pages 61-75

    8. Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells

      • Kellie J. Williams, Robert A. Godke, Kenneth R. Bondioli
      Pages 77-86

    9. Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells

      • Kevin C. Hicok, Marc H. Hedrick
      Pages 87-105

  3. Phenotyping Methods

    1. Front Matter

      Pages 107-107
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    2. Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

      • Stan Gronthos, Andrew C. W. Zannettino
      Pages 109-120

    3. Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry

      • H. Alan Tucker, Bruce A. Bunnell
      Pages 121-131

    4. Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells

      • Kevin R. McIntosh
      Pages 133-150

    5. Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

      • Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, Anne Bouloumié
      Pages 151-161

    6. Gel-Based and Gel-Free Proteomic Technologies

      • Peter Scherp, Ginger Ku, Liana Coleman, Indu Kheterpal
      Pages 163-190

  4. Differentiation Methods: In Vitro and In Vivo Applications

    1. Front Matter

      Pages 191-191
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    2. Adipogenic Differentiation of Adipose-Derived Stem Cells

      • Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Teddi Hebert, Yuan-Di C. Halvorsen, Benjamin M. Buehrer et al.
      Pages 193-200

    3. Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells

      • Bradley T. Estes, Farshid Guilak
      Pages 201-217

    4. Neural Differentiation of Human Adipose Tissue-Derived Stem Cells

      • Ji Min Yu, Bruce A. Bunnell, Soo-Kyung Kang
      Pages 219-231
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Keywords

About this book

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Reviews

From the reviews:

“This book is pleasing the reader … . Clear enough, the statistics is impressive … . I am quite sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will for sure appear more and more in the ISI statistics.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Editors and Affiliations

  • , Stem Cell Biology, Pennington Biomedical Research Center, Baton Rouge, USA

    Jeffrey M. Gimble

  • School of Medicine, Department of Pharmacology, Tulane University, New Orleans, USA

    Bruce A. Bunnell

Bibliographic Information

  • Book Title: Adipose-Derived Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Jeffrey M. Gimble, Bruce A. Bunnell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61737-960-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61737-959-8Published: 19 November 2010

  • Softcover ISBN: 978-1-4939-5781-1Published: 23 August 2016

  • eBook ISBN: 978-1-61737-960-4Published: 17 November 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 474

  • Topics: Cell Biology

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