Mesenchymal Stem Cell Assays and Applications

1. Front Matter

   Pages i-xvi

   PDF

2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. Mesenchymal Stem Cell Assays and Applications

      • Mohan C. Vemuri, Lucas G. Chase, Mahendra S. Rao

      Pages 3-8

3. Isolation and Expansion of MSCs from Various Sources

   1. Front Matter

      Pages 9-9

      PDF

   2. Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow

      • Patrice Penfornis, Radhika Pochampally

      Pages 11-21

   3. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis

      • Patrick Horn, Simone Bork, Wolfgang Wagner

      Pages 23-35

   4. Isolation and Growth of Adipose Tissue-Derived Stem Cells

      • Vladimir Zachar, Jeppe Grøndahl Rasmussen, Trine Fink

      Pages 37-49

   5. Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells

      • Alp Can, Deniz Balci

      Pages 51-62

   6. The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions

      • Anita Laitinen, Johanna Nystedt, Saara Laitinen

      Pages 63-73

   7. Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture

      • Justin D. Klein, Dario O. Fauza

      Pages 75-88

   8. Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells

      • Nina Ilic, Gary Brooke, Patricia Murray, Sarah Barlow, Tony Rossetti, Rebecca Pelekanos et al.

      Pages 89-106

   9. A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells

      • Stan Gronthos, Agnieszka Arthur, P. Mark Bartold, Songtao Shi

      Pages 107-121

   10. Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas

       • Karen L. Seeberger, Alana Eshpeter, Gregory S. Korbutt

       Pages 123-140

   11. Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells

       • Ruenn Chai Lai, Andre Choo, Sai Kiang Lim

       Pages 141-150

   12. Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells

       • Nance Beyer Nardi, Melissa Camassola

       Pages 151-160

   13. Cryopreservation and Revival of Mesenchymal Stromal Cells

       • Mandana Haack-Sørensen, Jens Kastrup

       Pages 161-174

   14. Dynamic Expansion Culture for Mesenchymal Stem Cells

       • Hicham Majd, Thomas M. Quinn, Pierre-Jean Wipff, Boris Hinz

       Pages 175-188

   15. Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers

       • Francisco dos Santos, Pedro Z. Andrade, Gemma Eibes, Cláudia Lobato da Silva, Joaquim M. S. Cabral

       Pages 189-198

4. MSC Lineage Differentiation and Analysis

   1. Front Matter

      Pages 199-199

      PDF

   2. Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells

      • Deepak M. Gupta, Nicholas J. Panetta, Michael T. Longaker

      Pages 201-214

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

• Human Cord Blood-Derived MSC
• Human Multipotent Stromal Cells
• MSC
• Marrow derived stromal cells

• , Primary and Stem Cell Systems, Life Technologies, Frederick, USA

  Mohan Vemuri

• Cellular Dynamics International, Inc., Madison, USA

  Lucas G. Chase

• Life Technologies, Frederick, USA

  Mahendra S. Rao

Policies and ethics