Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

1. Front Matter

   Pages i-xii

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2. Embryonic Stem Cells for Osteo-Degenerative Diseases

   • Nicole I. zur Nieden

   Pages 1-30

3. Methods for Culturing Mouse and Human Embryonic Stem Cells

   • Sabrina Lin, Prue Talbot

   Pages 31-56

4. Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells

   • Ludovic Vallier

   Pages 57-66

5. Functional Assays for Human Embryonic Stem Cell Pluripotency

   • Michael D. O’Connor, Melanie D. Kardel, Connie J. Eaves

   Pages 67-80

6. Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells

   • Helen Spencer, Maria Keramari, Christopher M. Ward

   Pages 81-94

7. Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters

   • Parinya Noisa, Alai Urrutikoetxea-Uriguen, Wei Cui

   Pages 95-106

8. Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives

   • Stephanie N. Rushing, Anthony W. Herren, Deborah K. Lieu, Ronald A. Li

   Pages 107-120

9. Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers

   • Ana Fernandes-Platzgummer, Maria Margarida Diogo, Cláudia Lobato da Silva, Joaquim M. S. Cabral

   Pages 121-134

10. Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology

    • Susanne Trettner, Alexander Seeliger, Nicole I. zur Nieden

    Pages 135-149

11. Methods for Embryoid Body Formation: The Microwell Approach

    • Dawn P. Spelke, Daniel Ortmann, Ali Khademhosseini, Lino Ferreira, Jeffrey M. Karp

    Pages 151-162

12. Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview

    • Peiman Hematti

    Pages 163-174

13. Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells

    • Andre Choo, Sai Kiang Lim

    Pages 175-182

14. Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the “Raclure” Method

    • Emmanuel N. Olivier, Eric E. Bouhassira

    Pages 183-193

15. Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes

    • Beatrice Kuske, Vuk Savkovic, Nicole I. zur Nieden

    Pages 195-215

16. Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds

    • Núria Marí-Buyé, Carlos E. Semino

    Pages 217-237

17. Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells

    • Motokazu Tsuneto, Toshiyuki Yamane, Shin-Ichi Hayashi

    Pages 239-253

18. Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield

    • Lesley A. Davis, Anke Dienelt, Nicole I. zur Nieden

    Pages 255-272

19. Identification of Osteoclasts in Culture

    • Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, Naoyuki Takahashi

    Pages 273-284

20. Analysis of Glycosaminoglycans in Stem Cell Glycomics

    • Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, Robert J. Linhardt

    Pages 285-300

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.

• Controllable bioreactor processes
• Degenerative cartilage & bone diseases
• ESCs
• Osteoblasts, osteoclasts, chondrocytes
• Osteodegenerative
• Regenerative medicine
• Skeletal cell types
• Skeletal tissue repair
• Skeletogenesis
• Tissue engineering

From the reviews:

“Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. … This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. … The authors provide excellent minireviews and great easy-to-follow research protocols. … The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases.” (Bruce A. Fenderson, Doody’s Review Service, June, 2011)

• Dept. of Cell Biology & Neuroscience, University of California at Riverside, Riverside, USA

  Nicole I. Nieden

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