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Small Molecule Microarrays

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Mahesh Uttamchandani

    1. , DSO National Laboratories, Defence Medical and Environmental Resear, Singapore, Singapore

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    You can also search for this editor in PubMed   Google Scholar

  2. Shao Q. Yao

    1. Faculty of Science, Depts. Chemistry & Biological Sciences, National University of Singapore, Singapore, Singapore

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Covers chemical libraries, peptide libraries, and carbohydrates with contributions from experts in the microarray field -Provides essential methods and skills learned through hands-on experience and feedback at the bench -Includes detailed notes highlighting tips on troubleshooting and avoiding known experimental pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 669)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Expanding World of Small Molecule Microarrays

    • Mahesh Uttamchandani, Shao Q. Yao
    Pages 1-15

  3. Construction of Photo-Cross-Linked Microarrays of Small Molecules

    • Naoki Kanoh
    Pages 17-22

  4. Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules

    • Hyang Yeon Lee, Seung Bum Park
    Pages 23-42

  5. Detecting Protein–Small Molecule Interactions Using Fluorous Small-Molecule Microarrays

    • Arturo J. Vegas, Angela N. Koehler
    Pages 43-55

  6. A Method for Small Molecule Microarray-Based Screening for the Rapid Discovery of Affinity-Based Probes

    • Haibin Shi, Mahesh Uttamchandani, Shao Q. Yao
    Pages 57-68

  7. Nanodroplet Chemical Microarrays and Label-Free Assays

    • Dhaval Gosalia, Scott L. Diamond
    Pages 69-78

  8. Nanodroplet Microarrays for High-Throughput Enzyme Screening

    • Kang L. D. Aw, Shao Q. Yao, Mahesh Uttamchandani
    Pages 79-93

  9. The Application of the Chemical Array for Biological Study

    • Isao Miyazaki, Siro Simizu, Hiroyuki Osada
    Pages 95-107

  10. Peptide Arrays with a Chip

    • Alexander Nesterov, Edgar Dörsam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Märkle, Felix Löffler et al.
    Pages 109-124

  11. In Situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon Nanowires

    • Gaëlle Piret, Hervé Drobecq, Rabah Boukherroub, Oleg Melnyk
    Pages 125-133

  12. In Situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens

    • Rémi Desmet, Eric Diesis, Hervé Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie et al.
    Pages 135-145

  13. Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection

    • Marina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna et al.
    Pages 147-160

  14. Antibody Signatures Defined by High-Content Peptide Microarray Analysis

    • Antonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, Mike Schutkowski
    Pages 161-172

  15. High Density Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysates

    • Alexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, Mike Schutkowski
    Pages 173-181

  16. A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates

    • Xiaoming Han, Yoshiki Katayama
    Pages 183-194

  17. Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications

    • Sungjin Park, Myung-Ryul Lee, Injae Shin
    Pages 195-208

  18. A Microarray-Based Method to Perform Nucleic Acid Selections

    • Olga Aminova, Matthew D. Disney
    Pages 209-224

  19. Back Matter

    Pages 225-228
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Keywords

About this book

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Editors and Affiliations

  • , DSO National Laboratories, Defence Medical and Environmental Resear, Singapore, Singapore

    Mahesh Uttamchandani

  • Faculty of Science, Depts. Chemistry & Biological Sciences, National University of Singapore, Singapore, Singapore

    Shao Q. Yao

Bibliographic Information

  • Book Title: Small Molecule Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Mahesh Uttamchandani, Shao Q. Yao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-845-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-844-7Published: 21 September 2010

  • Softcover ISBN: 978-1-4939-5815-3Published: 23 August 2016

  • eBook ISBN: 978-1-60761-845-4Published: 21 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 228

  • Topics: Biochemistry, general, Biomaterials, Materials Science, general, Microarrays

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