Overview
- Establishes protein structure, function, activity, and interrelationships by authors of peer reviewed articles published in respected journals
- Covers computational methods to evaluate protein stability and the resultant change upon introducing mutations
- Utilizes animal models to identify loci that genetically interact with human disease-causing point mutations
- Includes detailed methods on protein structure and function mutagenesis
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 634)
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Table of contents (30 protocols)
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Front Matter
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Mutagenesis in Various Microbial Backgrounds
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Front Matter
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PCR Mutagenesis
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Front Matter
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Protein Evolution Mutagenesis
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Front Matter
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Keywords
About this book
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
Reviews
From the reviews of the third edition:
“This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. … Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. … This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area.” (Omer Iqbal, Doody’s Review Service, November, 2010)Editors and Affiliations
Bibliographic Information
Book Title: In Vitro Mutagenesis Protocols
Book Subtitle: Third Edition
Editors: Jeff Braman
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-652-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-651-1Published: 12 July 2010
Softcover ISBN: 978-1-4939-5666-1Published: 23 August 2016
eBook ISBN: 978-1-60761-652-8Published: 30 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIV, 442
Topics: Genetic Engineering, Human Genetics, Proteomics