Overview
- Collective effort by nearly 70 scientists from over 20 leading laboratories worldwide to produce all-inclusive yet concise practical manual to peptidomics
- Complete: Includes all peptidomic methods in a single volume
- Comprehensive: Covers a wide range of species from bacterial to man
- Convenient: Provides step-by-step laboratory protocols with troubleshooting advice
- Captivating: Supplies the best practical introduction to the fascinating world of peptides
- Creative: Impresses with the thoroughness of the methods and the ingenuity of the experimental design
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 615)
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Table of contents (26 protocols)
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Front Matter
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Introduction
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Front Matter
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From Bacteria to Men
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Front Matter
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Keywords
About this book
Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
Reviews
From the reviews:
“Peptidomics–Methods and Protocols focuses on high-throughput techniques for the analysis of peptidomes from a wide range of biological systems … . The procedures covered in this book encompass a number of model and non-model organisms, and most chapters have been written by distinguished researchers who have made major contributions to their respective fields. Peptidomics–Methods and Protocols is an indispensable reference book for specialists, but also an introductory work for newcomers to the rapidly developing discipline of peptidomics.” (Juan J. Calvete, Analytical and Bioanalytical Chemistry, Vol. 398, September, 2010)
“The editor and co-authors of this volume must be congratulated on a comprehensive collection of very diverse peptidomics protocols that serve as an excellent introduction for a novice in this field. At the same time, the book provides the expert researcher with highly detailed experimental procedures and practical advice for the analysis of complex mixtures of peptides from a variety of sources. I warmly recommend this book to anyone interested in the field of peptidomics.” (Christian A. Olsen, ChemBioChem, Vol. 11, August, 2010)
Editors and Affiliations
Bibliographic Information
Book Title: Peptidomics
Book Subtitle: Methods and Protocols
Editors: Mikhail Soloviev
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-535-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-534-7Published: 22 December 2009
Softcover ISBN: 978-1-4939-5729-3Published: 23 August 2016
eBook ISBN: 978-1-60761-535-4Published: 15 December 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 395
Topics: Cell Biology, Protein Science, Analytical Chemistry, Proteomics