Overview
- Includes detailed description of cutting edge imaging techniques, including multiphoton microscopy, and software-based analysis of T cell infiltration in vivo
- Describes methods for setting up assays for the observation of lymphocyte migration under flow in vitro
- Provides description of effective genetic manipulation of the endothelium
- Includes assays for the investigation of recently described migratory mechanisms, including fugetaxis
Part of the book series: Methods in Molecular Biology (MIMB, volume 616)
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Table of contents (17 protocols)
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Front Matter
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Introductory Review
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Front Matter
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Migration of T Cells In Vitro
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Front Matter
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Migration of T Cells In Vivo
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Front Matter
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Monitoring T-Cell Migration in Human Diseases
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Front Matter
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Keywords
About this book
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.
Reviews
From the reviews:
“This book, as the title describes, is primarily a collection of methods to investigate T-cell trafficking. … this book details a comprehensive range of techniques for monitoring T-cell trafficking, along with the background behind the techniques, as well as their potential uses. This book would appeal to anyone interested in the field of T-cell trafficking and would be invaluable to PhD students and postdoctoral scientist setting up these techniques.” (Judith Ramage, Immunology News, August, 2011)Editors and Affiliations
Bibliographic Information
Book Title: T-Cell Trafficking
Book Subtitle: Methods and Protocols
Editors: Federica M. Marelli-Berg, Sussan Nourshargh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-461-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-460-9Published: 17 September 2010
Softcover ISBN: 978-1-4939-5704-0Published: 30 April 2017
eBook ISBN: 978-1-60761-461-6Published: 08 April 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 290
Topics: Immunology, Laboratory Medicine