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  • © 2010

Functional Glycomics

Methods and Protocols

Editors:

  • Includes the latest developments and emerging state-of-the-art techniques to determine the glycome function
  • Examines problem-driven strategy and methodology for Glycobiology researchers, both advanced and beginners, in academia and industry
  • Provides integrated analysis approach of whole sugar chains
  • Presents a practical manual to identify and monitor modifications and changes in glycomes for biomarker discovery and vaccines
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 600)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Functional Glycomics and Glycobiology: An Overview

    • Jianjun Li, James C. Richards
    Pages 1-8
  3. Historical Overview of Glycoanalysis

    • Alicia M. Bielik, Joseph Zaia
    Pages 9-30
  4. Quantitative Glycomics

    • Ron Orlando
    Pages 31-49
  5. Biotinylated Multivalent Glycoconjugates for Surface Coating

    • Alexander A. Chinarev, Oxana E. Galanina, Nicolai V. Bovin
    Pages 67-78
  6. In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit

    • Weiqing Han, Lei Li, Nicholas Pettit, Wen Yi, Robert Woodward, Xianwei Liu et al.
    Pages 93-110
  7. Glycoprotein Characterization

    • Susan M. Twine, Luc Tessier, John F. Kelly
    Pages 111-131
  8. N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry

    • S.O. Siu, Maggie P.Y. Lam, Edward Lau, William S.B. Yeung, David M. Cox, Ivan K. Chu
    Pages 133-143
  9. Microarray-Based Study of Carbohydrate–Protein Binding

    • Zhenxin Wang, Jingqing Gao
    Pages 145-153
  10. The Application of NMR Spectroscopy to Functional Glycomics

    • Jean-Robert Brisson, Evgeny Vinogradov, David J. McNally, Nam Huan Khieu, Ian C. Schoenhofen, Susan M. Logan et al.
    Pages 155-173
  11. Metabolomics in Glycomics

    • Evelyn C. Soo, Joseph P. M. Hui
    Pages 175-186
  12. Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry

    • Christopher W. Reid, Jacek Stupak, Christine M. Szymanski
    Pages 187-197
  13. A Glycomics Approach to the Discovery of Potential Cancer Biomarkers

    • Hyun Joo An, Carlito B. Lebrilla
    Pages 199-213
  14. N-Linked Protein Glycosylation in a Bacterial System

    • Harald Nothaft, Xin Liu, David J. McNally, Christine M. Szymanski
    Pages 227-243

About this book

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

Reviews

From the reviews:

“This book provides a useful introduction to the field of glycobiology as well as detailed protocols relating to methods of analyses. … relevance to the microbiologist who is considering exploring this field and wishes to gain an understanding of current methodologies.” (Sheila Patrick, Microbiology Today, 2010)

“The book consists of 19 chapters prepared by an international team of authors. … book will be very useful source for many researchers in glycobiology fields. It should be recommended also to students and teachers of colleges and universities who specialized in glycobiology. I would like to mention that the book is well done including a materials and methods part to each chapter, references, subject index, figures and tables.” (G. Ya. Wiederschain, Biochemistry, Vol. 75 (11), 2010)

Editors and Affiliations

  • Inst. Biological Sciences, National Research Council Canada, Ottawa, Canada

    Jianjun Li

Bibliographic Information

  • Book Title: Functional Glycomics

  • Book Subtitle: Methods and Protocols

  • Editors: Jianjun Li

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-454-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2010

  • Hardcover ISBN: 978-1-60761-453-1Published: 16 November 2009

  • Softcover ISBN: 978-1-4939-5697-5Published: 23 August 2016

  • eBook ISBN: 978-1-60761-454-8Published: 31 October 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 284

  • Topics: Biochemistry, general, Human Physiology, Carbohydrate Chemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access