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Liposomes

Methods and Protocols, Volume 2: Biological Membrane Models

  • Book
  • © 2010

Overview

Editors:
  1. Volkmar Weissig

    1. College of Pharmacy, Midwestern University, Glendale, U.S.A.

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  • Volume 2 specifically:
  • Provides an essential and comprehensive source for every investigator whose research involves any one aspect of lipids as essential components of biological membranes
  • Serves as a comprehensive compilation of cutting-edge methodologies centered around lipids as the essential components of biological membranes
  • With two volumes totaling 72 chapters, presents the first ever published comprehensive reference for the application of liposomes as pharmaceutical nanoscale drug delivery systems and as models for biological membranes
  • Provides in a very comprehensive way detailed guidance for the utilization of liposomes in Pharmacy, Chemistry, Biochemistry, Biophysics, and Molecular Biology
  • Offers a truly interdisciplinary approach to make the versatile features of liposomes accessible to a very wide range of investigators in Academia and Industry
  • Acts as a comprehensive guide to preparing, purifying, and physico-chemically characterizing all currently known types of liposomes
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 606)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Utilization of Liposomes for Studying Drug Transfer and Uptake

    • Alfred Fahr, Xiangli Liu
    Pages 1-10

  3. The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles

    • James R. Reed
    Pages 11-20

  4. Use of Liposomes to Study Cellular Osmosensors

    • Reinhard Krämer, Sascha Nicklisch, Vera Ott
    Pages 21-30

  5. Studying Mechanosensitive Ion Channels Using Liposomes

    • Boris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny Petrov, Prithwish Pal, Alexander Fook Weng Foo et al.
    Pages 31-53

  6. Studying Amino Acid Transport Using Liposomes

    • Cesare Indiveri
    Pages 55-68

  7. Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes

    • Chris T. Höfer, Andreas Herrmann, Peter Müller
    Pages 69-82

  8. Liposomal Reconstitution of Monotopic Integral Membrane Proteins

    • Zahra MirAfzali, David L. DeWitt
    Pages 83-94

  9. The Reconstitution of Actin Polymerization on Liposomes

    • Mark Stamnes, Weidong Xu
    Pages 95-103

  10. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions

    • L.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis A. Bagatolli et al.
    Pages 105-114

  11. Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles

    • Martin Stöckl, Jörg Nikolaus, Andreas Herrmann
    Pages 115-126

  12. Biosynthesis of Proteins Inside Liposomes

    • Pasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza, Pier Luigi Luisi
    Pages 127-145

  13. Study of Respiratory Cytochromes in Liposomes

    • Iseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, Katia C. U. Mugnol
    Pages 147-165

  14. Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity

    • Salette Reis, Marlene Lúcio, Marcela Segundo, José L. F. C. Lima
    Pages 167-188

  15. Studying Colloidal Aggregation Using Liposomes

    • Juan Sabín, Gerardo Prieto, Félix Sarmiento
    Pages 189-198

  16. Assessment of Liposome–Cell Interactions

    • Jan A. A. M. Kamps
    Pages 199-207

  17. Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells

    • Nejat Düzgüneş, Henrique Faneca, Maria C. Pedroso de Lima
    Pages 209-232

  18. The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes

    • David Miller, Paula J. Booth
    Pages 233-245

  19. Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling

    • Witold K. Subczynski, Marija Raguz, Justyna Widomska
    Pages 247-269

  20. Membrane Translocation Assayed by Fluorescence Spectroscopy

    • Jana Broecker, Sandro Keller
    Pages 271-289
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Keywords

About this book

Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.

Reviews

From the reviews:

“Provide a comprehensive guide to the preparation, characterisation, and potential use of currently known types of liposomes. They are written using an interdisciplinary approach, and so provide essential knowledge of liposomes, for not only researchers in this particular field, but also scientists in wider pharmaceutical and biological areas. … very inspiring – the potential for liposomes is immense – and these well-written chapters ignited notions of further applications – this is clearly a very exciting field.” (Trudy L. Knight, BTS Newsletter, Issue 37, Winter, 2010)

Editors and Affiliations

  • College of Pharmacy, Midwestern University, Glendale, U.S.A.

    Volkmar Weissig

Bibliographic Information

  • Book Title: Liposomes

  • Book Subtitle: Methods and Protocols, Volume 2: Biological Membrane Models

  • Editors: Volkmar Weissig

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-447-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2010

  • Hardcover ISBN: 978-1-60761-446-3Published: 23 December 2009

  • Softcover ISBN: 978-1-61779-687-6Published: 06 April 2012

  • eBook ISBN: 978-1-60761-447-0Published: 16 December 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 548

  • Number of Illustrations: 119 b/w illustrations, 7 illustrations in colour

  • Topics: Pharmacology/Toxicology, Cell Biology, Membrane Biology, Lipidology

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