Overview
- Orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics
- Introduces the fundamental resources and continues with the most recent molecular technologies
- Presents applicable techniques and functional assays utilizing specific small molecules
Part of the book series: Methods in Molecular Biology (MIMB, volume 577)
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Table of contents (20 protocols)
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The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics
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Modification of cDNA Resources Utilized for Reverse Chemical Genetics
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The Refinement of Established Methods by Recent Innovations
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Potential Applicable Techniques for Reverse Chemical Genetics
Keywords
About this book
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biologyâ„¢ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.
Editors and Affiliations
Bibliographic Information
Book Title: Reverse Chemical Genetics
Book Subtitle: Methods and Protocols
Editors: Hisashi Koga
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-232-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60761-231-5Published: 24 September 2009
Softcover ISBN: 978-1-61779-641-8Published: 04 May 2012
eBook ISBN: 978-1-60761-232-2Published: 29 August 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 272
Number of Illustrations: 60 b/w illustrations, 4 illustrations in colour
Topics: Human Genetics, Gene Function