Editors:
- Presents a collection of methods described by the experts in the field of DNA replication
- Contains overview chapters as an introduction to the field of DNA replication and as a guide in selecting the methods to use
- Covers techniques for various biological systems, including viral, bacterial, yeast and mammalian DNA replication
- Describes assays based on different technical approaches, including, among others, whole cell microscopy, genetics, in vitro biochemistry and DNA fiber analysis
- Provides detailed, step-by-step protocols for setting up a technique
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 521)
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Table of contents (36 protocols)
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Front Matter
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Methods for Analysis of Replication of a Single Locus
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Front Matter
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Genome-Wide Analysis Methods
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Front Matter
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About this book
Since the discovery of DNA structure and throughout the ensuing “DNA era”, the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.
Editors and Affiliations
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Marie Curie Research Institute, Oxted, United Kingdom
Sonya Vengrova, Jacob Z. Dalgaard
Bibliographic Information
Book Title: DNA Replication
Book Subtitle: Methods and Protocols
Editors: Sonya Vengrova, Jacob Z. Dalgaard
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-815-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-814-0Published: 01 April 2009
Softcover ISBN: 978-1-4939-5660-9Published: 23 August 2016
eBook ISBN: 978-1-60327-815-7Published: 05 April 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 701
Topics: Biochemistry, general, Genetic Engineering, Animal Genetics and Genomics, Biochemical Engineering