Editors:
- Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field
- Details the process of turning a biological observation into a high-throughput screen
- Covers a wide range of cell-based assay formats and systems
- Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis
- Comprehensive guide to developing, running, and analyzing cell-based assays in high-throughput screening
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 486)
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Table of contents (14 protocols)
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Front Matter
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Back Matter
About this book
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Reviews
From the reviews:
"‘Cell-based assays for high-throughput screening’ is a highly practical resource, written by experts in each of the techniques … . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. … aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)
Editors and Affiliations
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Broad Institute of Harvard, Chemical Biology Program, Massachusetts Institute of Technology, Cambridge, U.S.A.
Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner
Bibliographic Information
Book Title: Cell-Based Assays for High-Throughput Screening
Book Subtitle: Methods and Protocols
Editors: Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-545-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-544-6Published: 28 April 2009
Softcover ISBN: 978-1-62703-907-9Published: 27 November 2014
eBook ISBN: 978-1-60327-545-3Published: 05 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 218