Overview
- Editors:
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Preston B. Rich
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Dept. Surgery, University of North Carolina, Chapel Hill, U.S.A.
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Christelle Douillet
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Dept. Surgery, University of North Carolina, Chapel Hill, U.S.A.
- Provides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field
- Comprehensive guide to developing, validating, running, and analyzing bioluminescence assays
- Develops detection and imaging methods
- Describes the use of a wide variety of bioluminescence tools: various luciferases, reporter gene systems including PIN-G reporters, light emitting bacteria, smart probes, BRET, quantums dots, transgenic animals, etc.
- Presents protocols with enough details to be followed and adapted by scientific teams with no previous experience in bioluminescence
- Contains numerous notes about potential pitfalls and tips on how to avoid failures
- Presents cutting-edge material that will also interest experts in the field of bioluminescence
- Provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields: studies of cell trafficking, protein-protein interactions, intracellular signaling, apoptosis, RNAi knockdown, bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors
- Covers a wide range of sub-cellular and cell-based assays for in vitro and in vivo analysis as well as non invasive, in vivo, and real time assays and monitoring for animal models
- Also includes methods for high throughput analysis of animal models
- Includes supplementary material: sn.pub/extras
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Table of contents (20 protocols)
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- Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Aldo Roda
Pages 1-13
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- John Virostko, E. Duco Jansen
Pages 15-23
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- Lucia Seminario-Vidal, Eduardo R. Lazarowski, Seiko F. Okada
Pages 25-36
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- Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason
Pages 37-45
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- Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, Yasuteru Urano
Pages 47-62
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- Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner et al.
Pages 63-74
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- Xiaojuan Chen, Dixon B. Kaufman
Pages 75-85
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- Kitchener D. Wilson, Mei Huang, Joseph C. Wu
Pages 87-103
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- Akira Kanno, Yoshio Umezawa, Takeaki Ozawa
Pages 105-114
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- Kathryn E. Luker, Gary D. Luker
Pages 125-135
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- Siouxsie Wiles, Brian D. Robertson, Gad Frankel, Angela Kerton
Pages 137-153
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- Garrett R. Rettig, Kevin G. Rice
Pages 155-171
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- Akira Kanno, Takeaki Ozawa, Yoshio Umezawa
Pages 185-192
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- Jian Luo, Tony Wyss-Coray
Pages 193-202
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- Carlos Villalobos, María Teresa Alonso, Javier García-Sancho
Pages 203-214
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- Mélanie Robitaille, Isabelle Héroux, Alessandra Baragli, Terence E. Hébert
Pages 215-234
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- Lynn Mckeown, Vicky C. Jones, Owen T. Jones
Pages 235-248
About this book
Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing – a backyard tradition that has endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947,WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ‘‘The energy source for bioluminescence in an isolated system’’ in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer,thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring power of nature’s elegance. We have painstakingly harnessedapowerfultoolthatenablesustoseekadeeperunderstandingofthecomplex mechanisms underpinning so many vital biologic systems. This second edition of Methods in Molecular Biology’s Bioluminescence: Methods and Protocols serves as a re- ableandutilitariancompilationofthenewestandmostinnovativetechniquesthathave emerged in this rapidly expanding and progressively diverse field. We are indebted to the authors for their thoughtful contributions, inspired by their rigorous dedication to the science of bioluminescence, humbled by theunyielding supportofourcolleagues,andgratefulfortheopportunityprovidedusbyJohnandJan Walker.
Reviews
From the reviews of the second edition:
“This is a compilation of laboratory protocols dealing with diverse applications of bioluminescence in the biological sciences. … The book is written primarily for scientists working in the field of biomedical sciences. … It also provides a number of updated protocols and examples for practicing investigators interested in using bioluminescence in their research. … This second edition should prove to be a valuable companion to all researchers interested in bioluminescence.” (Ruben Mestril, Doody’s Review Service, December, 2009)
