Overview
- Serves as an easily accessible reference volume for all aspects of measurement of inositol phosphates and lipids contributed by front-line researchers in the field
- Combines in one volume methodologies for both inositol phosphates and lipids and the key enzymes which interconnect them
- Covers the new field of inositol pyrophosphates as well as several established areas in the lipid and phosphate arena
Part of the book series: Methods in Molecular Biology (MIMB, volume 645)
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Table of contents (14 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
Editors and Affiliations
Bibliographic Information
Book Title: Inositol Phosphates and Lipids
Book Subtitle: Methods and Protocols
Editors: Christopher J. Barker
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-175-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60327-174-5Published: 27 May 2010
Softcover ISBN: 978-1-61779-689-0Published: 01 July 2012
eBook ISBN: 978-1-60327-175-2Published: 20 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 230
Number of Illustrations: 54 b/w illustrations
Topics: Cell Biology, Cell Physiology