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C. elegans

Methods and Applications

  • Book
  • © 2006

Overview

Editors:
  1. Kevin Strange

    1. Department of Anesthesiology, Vanderbilt University Medical Center, Nashville
      Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville
      Department of Pharmacology, Vanderbilt University Medical Center, Nashville

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 351)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. An Overview of C. elegans Biology

    • Kevin Strange
    Pages 1-11

  3. Comparative Genomics in C. elegans, C. briggsae, and Other Caenorhabditis Species

    • Avril Coghlan, Jason E. Stajich, Todd W. Harris
    Pages 13-29

  4. WormBase

    • Todd W. Harris, Lincoln D. Stein
    Pages 31-50

  5. C. elegans Deletion Mutant Screening

    • Robert J. Barstead, Donald G. Moerman
    Pages 51-58

  6. Insertional Mutagenesis in C. elegans Using the Drosophila Transposon Mos1

    • Jean-Louis Bessereau
    Pages 59-73

  7. Single-Nucleotide Polymorphism Mapping

    • M. Wayne Davis, Marc Hammarlund
    Pages 75-92

  8. Creation of Transgenic Lines Using Microparticle Bombardment Methods

    • Vida Praitis
    Pages 93-107

  9. Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA

    • Lisa Timmons
    Pages 109-117

  10. Delivery Methods for RNA Interference in C. elegans

    • Lisa Timmons
    Pages 119-125

  11. Functional Genomic Approaches in C. elegans

    • Todd Lamitina
    Pages 127-138

  12. Assays for Toxicity Studies in C. elegans With Bt Crystal Proteins

    • Larry J. Bischof, Danielle L. Huffman, Raffi V. Aroian
    Pages 139-154

  13. Fluorescent Reporter Methods

    • Harald Hutter
    Pages 155-173

  14. Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans

    • Michael M. Francis, Andres Villu Maricq
    Pages 175-192

  15. Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis

    • Michael A. Miller
    Pages 193-201

  16. Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocytochemistry

    • Robby M. Weimer
    Pages 203-221

  17. Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants

    • Keith Nehrke
    Pages 223-239

  18. Automated Imaging of C. elegans Behavior

    • Christopher J. Cronin, Zhaoyang Feng, William R. Schafer
    Pages 241-251

  19. Intracellular Ca2+ Imaging in C. elegans

    • Rex A. Kerr, William R. Schafer
    Pages 253-264

  20. In Vitro Culture of C. elegans Somatic Cells

    • Kevin Strange, Rebecca Morrison
    Pages 265-273
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Keywords

About this book

Molecular biology has driven a powerful reductionist, or “molecule-c- tric,” approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of “naïve reductionism. ” Red- tionist methods will continue to be an essential element of all biological research efforts, but “naïve reductionism,” the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2–5). The “systems” of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or “emergent” properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.

Editors and Affiliations

  • Department of Anesthesiology, Vanderbilt University Medical Center, Nashville

    Kevin Strange

  • Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville

    Kevin Strange

  • Department of Pharmacology, Vanderbilt University Medical Center, Nashville

    Kevin Strange

Bibliographic Information

  • Book Title: C. elegans

  • Book Subtitle: Methods and Applications

  • Editors: Kevin Strange

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597451517

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-597-2Published: 15 August 2006

  • Softcover ISBN: 978-1-61737-668-9Published: 01 December 2010

  • eBook ISBN: 978-1-59745-151-2Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 292

  • Number of Illustrations: 42 b/w illustrations

  • Topics: Human Genetics

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