Overview
- Editors:
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Jose M. Guisan
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Institute of Catalysis, CSIC, Campus UAM-Cantoblanco, Madrid, Spain
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Table of contents (37 protocols)
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- Beatriz M. Brena, Francisco Batista-Viera
Pages 15-30
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- Roger A. Sheldon, Rob Schoevaart, Luuk M. van Langen
Pages 31-45
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- Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan et al.
Pages 47-55
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- Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente et al.
Pages 57-64
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- Mónica Campás, Jean-Louis Marty
Pages 77-85
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- Ipsita Roy, Munishwar N. Gupta
Pages 87-95
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- Silvana Andreescu, Bogdan Bucur, Jean-Louis Marty
Pages 97-106
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- Ipsita Roy, Munishwar N. Gupta
Pages 107-116
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- Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan et al.
Pages 117-128
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- Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo et al.
Pages 129-141
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- Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente et al.
Pages 143-152
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- Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes et al.
Pages 153-162
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- Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor et al.
Pages 163-173
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- Alankar Vaidya, Lutz Fischer
Pages 175-183
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- Francisco Batista-Viera, Karen Ovsejevi, Carmen Manta
Pages 185-204
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- Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego et al.
Pages 205-216
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- Martina Koneracká, Peter Kopčanský, Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan et al.
Pages 217-228
About this book
Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Reviews
"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology
From Reviews of the First Edition...
"...enables researchers to rapidly enter the world of immobilization...an excellent reference work that will have long lasting value." -Quarterly Review of Biology
"...one of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today
"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology
"...an ideal reference book to have around the laboratory." -Trends in Cell Biology
"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal
"...the coverage of the various techniques is excellent..." -The Analyst
Editors and Affiliations
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Institute of Catalysis, CSIC, Campus UAM-Cantoblanco, Madrid, Spain
Jose M. Guisan
