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Food-Borne Pathogens

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Catherine C. Adley

    1. Microbiology Laboratory Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

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Part of the book series: Methods in Biotechnology (MIBT, volume 21)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. The Bacteria

    1. Front Matter

      Pages 1-1
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    2. Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources

      • Rosabel Falcón, Tatiana d’Albuquerque, Maria das Graças de Luna, Angela Corrêa de Freitas-Almeida, Tomomasa Yano
      Pages 3-13

    3. Detection and Purification of Bacillus cereus Enterotoxins

      • Toril Lindbäck, Per Einar Granum
      Pages 15-26

    4. Campylobacter

      • Rachel Gorman, Catherine C. Adley
      Pages 27-35

    5. Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces

      • Miia Lindström, Mari Nevas, Hannu Korkeala
      Pages 37-45

    6. Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex

      • Peter C. H. Feng, Steven R. Monday
      Pages 47-55

    7. PulseNet’s Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis

      • Lewis M. Graves, Balasubramanian Swaminathan
      Pages 57-72

    8. Plesiomonas shigelloides

      • Ivan Ciznar, Carlos González-Rey, Karel Krovacek, Anna Hostacka
      Pages 73-80

    9. Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies

      • Rachel Gorman, Catherine C. Adley
      Pages 81-90

    10. Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus

      • Moira M. Brett
      Pages 91-98

    11. Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples

      • Keya De, Ranjan K. Nandy, G. Balakrish Nair
      Pages 99-124

    12. HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes

      • Jennifer Colford
      Pages 125-131

  3. The Viruses

    1. Front Matter

      Pages 133-133
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    2. Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR

      • Christian M. Beuret
      Pages 135-152

    3. Detection of Enteroviruses

      • Miguel-Angel Jiménez-Clavero, Victoria Ley, Nuria Gómez, Juan-Carlos Sáiz
      Pages 153-169

    4. Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

      • Julie Jean, Ismaïl Fliss
      Pages 171-187

  4. The Parasites

    1. Front Matter

      Pages 189-189
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    2. Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis

      • Sandra M. O’Neill, Grace Mulcahy, John P. Dalton
      Pages 191-201

    3. Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish

      • Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, John E. Moore
      Pages 203-215
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Keywords

About this book

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Microbiology Laboratory Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

    Catherine C. Adley

Bibliographic Information

  • Book Title: Food-Borne Pathogens

  • Book Subtitle: Methods and Protocols

  • Editors: Catherine C. Adley

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/1592599907

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-465-4Published: 06 October 2005

  • Softcover ISBN: 978-1-61737-585-9Published: 19 November 2010

  • eBook ISBN: 978-1-59259-990-5Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XII, 268

  • Topics: Microbiology

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