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Molecular Diagnosis of Cancer

Methods and Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Joseph E. Roulston

    1. Division of Reproductive and Developmental Sciences, The University of Edinburgh, The Royal Infirmary, Edinburgh, Scotland, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. John M. S. Bartlett

    1. Division of Cancer and Molecular Pathology, University Department of Surgery, Glasgow Royal Infirmary, Glasgow, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 97)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Prognostic and Predictive Factors

    • Michael Scott, Peter A. Hall
    Pages 1-11

  3. Assessment of Predictive Values of Tumor Markers

    • Joseph E. Roulston
    Pages 13-27

  4. Quality Assurance of Predictive Markers in Breast Cancer

    • Anthony Rhodes, Diana M. Barnes
    Pages 29-57

  5. Extraction of Nucleic Acid Templates

    • John M. S. Bartlett, Helen Speirs
    Pages 59-70

  6. Microdissection and Extraction of DNA From Archival Tissue

    • Joanne Edwards, James J. Going, John M. S. Bartlett
    Pages 71-75

  7. Fluorescence In Situ Hybridization

    • John M. S. Bartlett
    Pages 77-87

  8. HER2 FISH in Breast Cancer

    • John M. S. Bartlett, Amanda Forsyth
    Pages 89-101

  9. Fluorescence In Situ Hybridization for BCR-ABL

    • Mark W. Drummond, Elaine K. Allan, Andrew Pearce, Tessa L. Holyoake
    Pages 103-116

  10. UroVysion™ Multiprobe FISH in Urinary Cytology

    • Lukas Bubendorf, Bruno Grilli
    Pages 117-128

  11. Chromogenic In Situ Hybridization in Tumor Pathology

    • Jorma Isola, Minna Tanner
    Pages 133-144

  12. Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia

    • Marie Jarosova
    Pages 145-157

  13. Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization

    • Suzanne D. Vernon, Elizabeth R. Unger
    Pages 159-180

  14. A Nested RT-PCR Assay to Detect BCR/abl

    • Linda M. Wasserman
    Pages 181-189

  15. TP53 Mutation Detection by SSCP and Sequencing

    • Jenni Hakkarainen, Judith A. Welsh, Kirsi H. Vähäkangas
    Pages 191-208

  16. PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies

    • Jean Benhattar, Sandra Gebhard
    Pages 209-216

  17. Circulating DNA Analysis

    • Kwan-Chee Allen Chan, Yuk-Ming Dennis Lo
    Pages 217-236

  18. Microsatellite Instability

    • Gillian Gifford, Robert Brown
    Pages 237-250

  19. Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disorders

    • Jeremy M. E. Taylor, Peter H. Kay, Dominic V. Spagnolo
    Pages 251-265

  20. Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia

    • Maiko Matsushita, Rie Yamazaki, Yutaka Kawakami
    Pages 267-275
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About this book

We are currently experiencing a fundamental shift in the way in which we approach the characterization of cancer. Never before has the make up of cancer tissues and individual cells been so exhaustively researched and char- terized. We are now capable of producing molecular “fingerprints” that ch- acterize the expression of all known and unknown genes within tumors and their surrounding tissues. More than 30,000 different genes may be measured in each patient’s tumor in a single experiment. Simultaneously, novel therapies that exploit the molecular roadmap have been developed and are now being offered to patients. These novel agents, such as Glivec, Herceptin, Iressa, and others, specifically target individual genes within tumors and can produce d- matic responses in some patients. These drugs are only the forerunners of a coming tidal wave of novel therapeutics that individually target specific m- ecules within cancer cells—more than 300 such agents are currently in phase I or II clinical trials. This is an exciting time for cancer specialists and patients alike. However, if we have learned anything from the past 50 or more years of research into cancer, it is that Lord Beaverbrook, in founding the British national health service in the 1950s, was frighteningly prescient when he defined the primary goal of health care to be “Diagnosis, Diagnosis, Diag- sis. ” Now, more than ever, it is essential that appropriate diagnostic methods and approaches are applied to the selection of patients for treatment.

Reviews

"...concise and uniformly well written...contains a large compilation of applications of nucleic acid technologies that are in use or may be in use in the near future to diagnose and aid in the management of malignant diseases." - Doody's Health Sciences Book Review Journal

Review of the First Edition
The techniques described here are standard laboratory lore;the added value are the notes and tips on tricky details andoften overlooked precautions....Laboratory scientists...will get their money's worth. -Modern Pathology

"....a diverse collection of readily reproducible methods...the protocols presented follow step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls." - Tumori

From the reviews of the second edition:

"Dramatic advances in molecular biology over the past decades have led to an unprecedented understanding of cancer at a very detailed molecular level. … tips on troubleshooting are presented in each chapter, written by experts in the field. Given the amount of detail incorporated in each protocol, these should be readily reproducible and enable laboratories with no prior experience in a specific technique to master it and be able to use todays most powerful techniques in the molecular diagnosis of cancer." (Janis Racevskis, Medical Oncology, Vol. 22 (3), 2004)

Editors and Affiliations

  • Division of Reproductive and Developmental Sciences, The University of Edinburgh, The Royal Infirmary, Edinburgh, Scotland, UK

    Joseph E. Roulston

  • Division of Cancer and Molecular Pathology, University Department of Surgery, Glasgow Royal Infirmary, Glasgow, Scotland, UK

    John M. S. Bartlett

Bibliographic Information

  • Book Title: Molecular Diagnosis of Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Joseph E. Roulston, John M. S. Bartlett

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592597602

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-160-8Published: 15 March 2004

  • Softcover ISBN: 978-1-61737-396-1Published: 10 November 2010

  • eBook ISBN: 978-1-59259-760-4Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 2

  • Number of Pages: XII, 394

  • Topics: Pathology

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