Overview
- Editors:
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Miep H. Helfrich
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University of Aberdeen Medical School, Aberdeen, UK
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Stuart H. Ralston
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University of Aberdeen Medical School, Aberdeen, UK
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Table of contents (31 protocols)
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Culture of Osteolblasts and Osteocytes
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- Astrid Bakker, Jenneke Klein-Nulend
Pages 19-28
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- Andrew Scutt, Lynsey Reading, Nanette Scutt, Karen Still
Pages 29-39
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- Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, Jenneke Klein-Nulend
Pages 41-50
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Culture of Osteoclasts
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- Astrid Hoebertz, Timothy R. Arnett
Pages 53-64
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- Patricia Collin-Osdoby, Fred Anderson, Philip Osdoby
Pages 65-88
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- Fraser P. Coxon, Julie C. Frith, Helena L. Benford, Michael J. Rogers
Pages 89-99
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- Afsie Sabokbar, Nicholas S. Athanasou
Pages 101-111
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- Adrienne M. Flanagan, Helen M. Massey
Pages 113-128
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- Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, Tatsuo Suda
Pages 129-144
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- Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, Philip Osdoby
Pages 153-166
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Bone Organ Culture
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Front Matter
Pages 167-167
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- Michael J. Marshall, Marit N. Rowlands
Pages 169-182
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Histological and Ultrastructural Techniques
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Front Matter
Pages 199-199
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- Andrew P. Mee, Judith A. Hoyland
Pages 201-223
About this book
The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
Reviews
"Bone Research Protocols is a most welcome compilation of methods. In it, many of the most productive laboratories in some of the most challenging areas of cell biology reveal detailed protocols that have been used to solve key problems. Short of spending a sabbatical in one of the eminent laboratories that contributed, this volume provides the best possible introduction to these methods." - TRENDS in Endocrinology and Metabolism
