Overview
- Editors:
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Sharona Even-Ram
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Gene Therapy Inst., Hadassah Univ. Hospital, Jerusalem, Israel
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Vira Artym
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Lombardi Comprehensive Cancer Ctr., Georgetown University, Washington, U.S.A.
- A comprehensive protocol book featuring cutting-edge methods and techniques to study molecular, cellular, biochemical, and biophysical aspects of the extracellular matrix
- Bench-tested in-vivo, ex-vivo and in-vitro methods written by experts who provided detailed step-by-step instructions and trouble-shooting
- Easy-to-follow techniques that ensure reproducibility and save time and resources
- Valuable protocols that can be easily adapted and further modified to fit the individual needs of research teams
- Includes supplementary material: sn.pub/extras
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Table of contents (24 protocols)
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Molecular Biology
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- Vitali Alexeev, Olga Igoucheva
Pages 3-14
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- Mara Brancaccio, Emila Turco, Emilio Hirsch
Pages 15-50
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- Neil Smyth, Uwe Odenthal, Barbara Merkl, Mats Paulsson
Pages 63-72
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- David Staunton, Christopher J. Millard, A. Radu Aricescu, Iain D Campbell
Pages 73-99
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- Nicholas C. Avery, Trevor J. Sims, Allen J. Bailey
Pages 103-121
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Biochemical and biophysical analysis
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- Nigel W. Hodson, Cay M. Kielty, Michael J. Sherratt
Pages 123-141
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- Gradimir N. Misevic, Yannis Karamanos, Nikola J. Misevic
Pages 143-150
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- Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, David F. Holmes
Pages 151-161
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- Gradimir N. Misevic, Bernard Rasser, Vic Norris, Cédric Dérue, David Gibouin, Fabrice Lefebvre et al.
Pages 163-173
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- Michael J. Sherratt, Roger S. Meadows, Helen K. Graham, Cay M. Kielty, David F. Holmes
Pages 175-181
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- Emanuele Ostuni, George M. Whitesides, Donald E. Ingber, Christopher S. Chen
Pages 183-194
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Cell biology assays
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- Vira V. Artym, Kenneth M. Yamada, Susette C. Mueller
Pages 211-219
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- Paola Spessotto, Katia Lacrima, Pier Andrea Nicolosi, Eliana Pivetta, Martina Scapolan, Roberto Perris
Pages 221-250
About this book
The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade. Although well appreciated, the structural and biochemical complexity and the dynamic nature of the living matrix are still under extensive inv- tigation, yielding a growing number of methods with varying degree of sophistication and intricacy. In this edition of Extracellular Matrix Protocols, we compiled a variety of methods that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are indeed available in a limited number of centers, but are well worth the awareness and exposure to the ECM research community. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. All chapters were contributed by scientists who developed the methods or mastered and perfected methods that were routinely used in their laboratories. An effort was made to provide practical working details and helpful notes for the nonexpert user in order to assist reproducibility and accuracy. We hope that these valuable protocols will become helpful tools for ECM researchers and will be further developed and tailored to the s- cific needs of a growing number of applications.
Reviews
From the reviews of the second edition:
“Significant advances in the field of extracellular matrix during the past decade and current extensive investigations in this field have necessitated the second edition of this book on extracellular matrix protocols. … well illustrated with of figures, photographs, and graphs in color and in black-and-white. … students interested in cell biology will find this book quite useful. … this second edition is surely welcome in the research community. Both expert and nonexpert researchers in the field of extracellular matrix will find this book extremely useful.” (Omer Iqbal, Doody’s Review Service, July, 2009)
Editors and Affiliations
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Gene Therapy Inst., Hadassah Univ. Hospital, Jerusalem, Israel
Sharona Even-Ram
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Lombardi Comprehensive Cancer Ctr., Georgetown University, Washington, U.S.A.
Vira Artym
