Overview
- Includes coverage of a wide range of methodologies to study G-quadruplex structures
- Provides detailed protocols which ensure successful re-recreation of experimental procedures
- Supplies step-by-step instructions in order to enable researchers to set up their own high throughput screens for G-quadruplex ligands
- Explores computational approaches for the detection of G-quadruplex-forming structures
- Examines state of the art physical, chemical, and biological methods
- Contains tricks of the trade and valuable troubleshooting advice
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 608)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In "G-Quadruplex DNA: Methods and Protocols", experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, "G-Quadruplex DNA: Methods and Protocols "promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
Reviews
From the reviews:
“The book G-Quadruplex DNA … provides an excellent collection of important methods for the study of the DNA G-quadruplex structures. … I definitely recommend this book, which can be considered an outstanding source of information that addresses several problems … in the investigation of G-quadruplex structures. The book is appropriate for everyone working in the area of G-quadruplex research, including biologists, biochemists and chemists since it is a worthy guide for both beginners and experienced scientists interested in studying this increasingly attractive field.” (Aldo Galeone, ChemBioChem, Issue 11, September, 2010)Editors and Affiliations
Bibliographic Information
Book Title: G-Quadruplex DNA
Book Subtitle: Methods and Protocols
Editors: Peter Baumann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-363-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-58829-950-5Published: 18 December 2009
Softcover ISBN: 978-1-4939-5639-5Published: 23 August 2016
eBook ISBN: 978-1-59745-363-9Published: 10 December 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 276
Topics: Biochemistry, general, Cell Biology, Human Genetics