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Alcohol

Methods and Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Laura E. Nagy

    1. Departments of Gastroenterology and Pathobiology Cleveland Clinic and Department of Nutrition, Case Western Reserve University, Cleveland, USA

    View editor publications

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  • Step-by-step protocols for the most commonly used models of acute and chronic ethanol exposure
  • Detailed procedures for cell culture experiments with ethanol
  • Comprehensive methods for studies of fetal alcohol syndrome (FAS) including Zebrafish, avian embryo and rodent models
  • Up-to-date proteomics and micro-array protocols
  • Specific protocols of interest to neuroscientists, immunologists, physiologists, cell biologists and biochemists
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 447)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Animal Models of Acute and Chronic Ethanol Exposure

    1. Acute Models of Ethanol Exposure to Mice

      • Timothy P. Plackett, Elizabeth J. Kovacs
      Pages 3-9

    2. A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury

      • George L. Tipoe, Emily C. Liong, Tung-Ming Leung, Amin A. Nanji
      Pages 11-31

    3. Intragastric Ethanol Infusion Model in Rodents

      • Hide Tsukamoto, Hasmik Mkrtchyan, Alla Dynnyk
      Pages 33-48

    4. A Practical Method of Chronic Ethanol Administration in Mice

      • Ruth A. Coleman, Betty M. Young, Lucas E. Turner, Robert T. Cook
      Pages 49-59

  3. Animal Models of Ethanol Exposure During Development

    1. Analysis of Ethanol Developmental Toxicity in Zebrafish

      • Robert L. Tanguay, Mark J. Reimers
      Pages 63-74

    2. The Avian Embryo in Fetal Alcohol Research

      • Susan M. Smith
      Pages 75-84

    3. Artificial Rearing

      • Hector D. Dominguez, Jennifer D. Thomas
      Pages 85-100

    4. Intragastric Intubation of Alcohol During the Perinatal Period

      • Sandra J. Kelly, Charles R. Lawrence
      Pages 101-110

  4. Cell Culture Approaches to Studying Ethanol Exposure

    1. Human Monocytes, Macrophages, and Dendritic Cells: Alcohol Treatment Methods

      • Gyongyi Szabo, Pranoti Mandrekar
      Pages 113-124

    2. Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol

      • Amanda Lindke, Barbara Tremper-Wells, Michael W. Miller
      Pages 125-136

    3. Development and Properties of HepG2 Cells That Constitutively Express CYP2E1

      • Defeng Wu, Arthur I. Cederbaum
      Pages 137-150

    4. Modeling the Impact of Alcohol on Cortical Development in a Dish: Strategies from Mapping Neural Stem Cell Fate

      • Rajesh C. Miranda, Daniel R. Santillano, Cynthia Camarillo, Douglas Dohrman
      Pages 151-168

  5. Regulation of Specific Organ Systems in Response to Ethanol

    1. Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer

      • R. K. Rao
      Pages 171-183

    2. Alcohol-Induced Oxidative Stress in the Liver: In Vivo Measurements

      • Gavin E. Arteel
      Pages 185-197

    3. Isolation of Kupffer Cells from Rats Fed Chronic Ethanol

      • Megan R. McMullen, Michele T. Pritchard, Laura E. Nagy
      Pages 199-212

    4. Dendritic Cells in Chronic In Vivo Ethanol Exposure Models

      • Kevin L. Legge, Annette J. Schlueter
      Pages 213-233

    5. Formation and Immunological Properties of Aldehyde-derived Protein Adducts following Alcohol Consumption

      • Geoffrey M. Thiele, Lynell W. Klassen, Dean J. Tuma
      Pages 235-257

    6. Assessment of Natural Killer (NK) and NKT Cells in Murine Spleens and Livers

      • Michael R. Shey, Zuhair K. Ballas
      Pages 259-276

    7. Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets

      • Betty M. Young, Susan Wiechert, Ruth A. Coleman, Prajwal Gurung, Robert T. Cook
      Pages 277-294
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Keywords

About this book

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Editors and Affiliations

  • Departments of Gastroenterology and Pathobiology Cleveland Clinic and Department of Nutrition, Case Western Reserve University, Cleveland, USA

    Laura E. Nagy

Bibliographic Information

  • Book Title: Alcohol

  • Book Subtitle: Methods and Protocols

  • Editors: Laura E. Nagy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-242-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-906-2Published: 07 March 2008

  • Softcover ISBN: 978-1-61737-837-9Published: 19 November 2010

  • eBook ISBN: 978-1-59745-242-7Published: 15 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 420

  • Number of Illustrations: 72 b/w illustrations, 2 illustrations in colour

  • Topics: Pharmacology/Toxicology, Human Physiology, Pathology, Molecular Medicine, Cell Biology

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