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  • © 2008

Gene Therapy Protocols

Volume 1: Production and In Vivo Applications of Gene Transfer Vectors

Authors:

  • Collects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field
  • Covers current and emerging methods for production of viral and non-viral gene transfer vectors
  • Provides detailed protocols for applications in stem cell biology, cancer research and infectious disease

Part of the book series: Methods in Molecular Biology (MIMB, volume 433)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Production of Retroviral Vectors for Clinical Use

    • Kenneth Cornetta, Lilith Reeves, Scott Cross
    Pages 17-32
  3. Large-Scale Production of Recombinant Adeno-Associated Viral Vectors

    • Alejandro Negrete, Robert M. Kotin
    Pages 79-96
  4. Construction and Production of Recombinant Herpes Simplex Virus Vectors

    • William F. Goins, David M. Krisky, James B. Wechuck, Shaohua Huang, Joseph C. Glorioso
    Pages 97-103
  5. Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation

    • Jonathan D., Schertzer and, Gordon S. Lynch
    Pages 115-125
  6. Chitosan Nanoparticle-Mediated Gene Transfer

    • Dongwon Lee, Shyam S. Mohapatra
    Pages 127-140
  7. PEG–PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues

    • Gordon J. Lutz, Shashank R. Sirsi, Jason H. Williams
    Pages 141-150
  8. Non-Viral Gene Delivery with Cationic Liposome–DNA Complexes

    • Kai K. Ewert, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, Cyrus R. Safinya
    Pages 159-175
  9. Applications of Lentiviral Vectors in Noninvasive Molecular Imaging

    • Abhijit De, Shahriar Shah Yaghoubi, Sanjiv Sam Gambhir
    Pages 177-202
  10. Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells

    •  Lucienne M. Ide, Elisabeth Javazon, H. Trent Spencer
    Pages 213-228
  11. Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo

    • Uta Griesenbach, Felix M. Munkonge, Stephanie Sumner-Jones, Emma Holder, Stephen N. Smith, A. Christopher Boyd et al.
    Pages 229-242
  12. Oncolytic Adenoviruses for Cancer Gene Therapy

    • Ta-Chiang Liu, Stephen H. Thorne, David H. Kirn
    Pages 243-258
  13. Nanoparticle-Mediated Gene Delivery to the Lung

    • Rajagopal Ramesh
    Pages 301-332

About this book

In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Authors and Affiliations

  • Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta

    Joseph M. Le Doux

Bibliographic Information

  • Book Title: Gene Therapy Protocols

  • Book Subtitle: Volume 1: Production and In Vivo Applications of Gene Transfer Vectors

  • Authors: Joseph M. Le Doux

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-237-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-903-1Published: 24 April 2008

  • Softcover ISBN: 978-1-62703-956-7Published: 02 November 2014

  • eBook ISBN: 978-1-59745-237-3Published: 05 August 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 386

  • Topics: Human Genetics, Animal Genetics and Genomics, Gene Therapy

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access