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Macromolecular Crystallography Protocols, Volume 2

Structure Determination

  • Book
  • © 2007

Overview

Editors:
  1. Sylvie Doublié

    1. Department of Microbiology and Molecular Genetics, University of Vermont, Burlingon

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Part of the book series: Methods in Molecular Biology (MIMB, volume 364)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
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  2. Cryocrystallography of Macromolecules

    • Elspeth Garman, Robin L. Owen
    Pages 1-18

  3. Determination of Reaction Intermediate Structures in Heme Proteins

    • Kelvin Chu
    Pages 19-29

  4. Annealing Macromolecular Crystals

    • B. Leif Hanson, Gerard J. Bunick
    Pages 31-41

  5. First Analysis of Macromolecular Crystals

    • David Jeruzalmi
    Pages 43-61

  6. X-Ray Data Collection From Macromolecular Crystals

    • Elspeth Garman, Robert M. Sweet
    Pages 63-93

  7. Characterizing a Crystal From an Initial Native Dataset

    • Michael R. Sawaya
    Pages 95-120

  8. Molecular Replacement

    • Eric A. Toth
    Pages 121-147

  9. Phase Determination Using Halide Ions

    • Miroslawa Dauter, Zbigniew Dauter
    Pages 149-158

  10. The Same But Different

    • Mark A. Rould
    Pages 159-181

  11. Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement With Anomalous Scattering Data Using Shake-and-Bake

    • G. David Smith, Christopher T. Lemke, P. Lynne Howell
    Pages 183-195

  12. Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments

    • Ralf W. Grosse-Kunstleve, Thomas R. Schneider
    Pages 197-214

  13. Automated Structure Solution With autoSHARP

    • Clemens Vonrhein, Eric Blanc, Pietro Roversi, Gérard Bricogne
    Pages 215-230

  14. Introduction to Macromolecular Refinement

    • Dale E. Tronrud
    Pages 231-253

  15. Quality Control and Validation

    • Gerard J. Kleywegt
    Pages 255-272

  16. Crystallographic Software

    • Stephen J. Everse, Sylvie Doublié
    Pages 273-278

  17. Back Matter

    Pages 279-283
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Keywords

About this book

In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Editors and Affiliations

  • Department of Microbiology and Molecular Genetics, University of Vermont, Burlingon

    Sylvie Doublié

Bibliographic Information

  • Book Title: Macromolecular Crystallography Protocols, Volume 2

  • Book Subtitle: Structure Determination

  • Editors: Sylvie Doublié

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597452661

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-902-4Published: 05 February 2007

  • Softcover ISBN: 978-1-61737-836-2Published: 19 November 2010

  • eBook ISBN: 978-1-59745-266-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 284

  • Number of Illustrations: 70 b/w illustrations

  • Topics: Cell Biology

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