Overview
- 23 state-of-the-art, widely applicable protocols with minute detail
- Written by international experts from 20 institutions
- Relevant to a wide array of professions
Part of the book series: Methods in Molecular Biology (MIMB, volume 454)
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Table of contents (23 protocols)
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Front Matter
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Detection and Discovery of Coronaviruses
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Front Matter
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Isolation, Growth, Titration, and Purification of Coronaviruses
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Front Matter
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Structure of Covs Analyzed by Electron Microscopy
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Front Matter
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Expression of Coronavirus Proteins and Crystallization
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Front Matter
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Raising Antibodies to Coronavirus Proteins
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Keywords
About this book
In 2003, the word “coronavirus” spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors.
Comprehensive and cutting-edge, SARS- and Other Coronaviruses Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.
Reviews
From the reviews:
"The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening consequences in humans. ... this book describes the laboratory techniques used to study them. ... Scientists and students working in virology laboratories and, specifically, laboratories working with coronaviruses are the intended audience. ... each chapter contains a copious amount of detailed information as well as specific notes with technical advice. This is a valuable resource for scientists working with coronaviruses." (Rebecca T. Horvat, Doody's Review Service, November, 2008)
"This is volume 454 in the series Methods in Molecular Biology. … if you work on coronaviruses, or want to enter the field, this is a must-have book at an affordable price." (Stuart Siddell, Microbiology Today, February, 2009)
Editors and Affiliations
Bibliographic Information
Book Title: SARS- and Other Coronaviruses
Book Subtitle: Laboratory Protocols
Editors: Dave Cavanagh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-181-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-867-6Published: 02 September 2008
Softcover ISBN: 978-1-61737-815-7Published: 19 November 2010
eBook ISBN: 978-1-59745-181-9Published: 05 December 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 326
Number of Illustrations: 64 b/w illustrations
Topics: Medical Microbiology, Infectious Diseases, Virology, Laboratory Medicine