Editors:
- Contains simple and complete protocols for culturing osteoblasts and osteoclasts
- Explains how to utilize various rodent models to study and treat osteoporosis
- Provides protocols for increasing or decreasing gene expression in bone cells
- Examines molecular, genetic and imaging techniques to study bone
- Covers multiple protocols to study mechanical loading on bone and its cells
- Each chapter contains troubleshooting tips from respected experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 455)
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Table of contents (23 protocols)
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Front Matter
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In Vivo Rodent Models
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Molecular and Genetic Techniques
About this book
In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.
Editors and Affiliations
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Department of Orthopedic Surgery, Mayo Clinic, Rochester, USA
Jennifer J. Westendorf
Bibliographic Information
Book Title: Osteoporosis
Book Subtitle: Methods and Protocols
Editors: Jennifer J. Westendorf
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-104-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-828-7Published: 16 April 2008
Softcover ISBN: 978-1-62703-847-8Published: 03 November 2014
eBook ISBN: 978-1-59745-104-8Published: 08 May 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIX, 354
Topics: Orthopedics, Molecular Medicine, Cell Biology, Imaging / Radiology