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  • © 2007

Patch-Clamp Analysis

Advanced Techniques

Editors:

  • Provides readily reproducible, step-by-step protocols that ensure experimental success
  • Describes methods combining patch-clamp with molecular biology and imaging
  • Includes new state-of-the-art techniques
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 38)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Technology of Patch-Clamp Electrodes

    • Richard A. Levis, James L. Rae
    Pages 1-34
  3. Whole-Cell Patch-Clamp Recordings

    • Harald Sontheimer, Michelle L. Olsen
    Pages 35-68
  4. Single-Channel Recording

    • David J.A. Wyllie
    Pages 69-119
  5. Combined Fluorometric and Electrophysiological Recordings

    • Hartmut Schmidt, Jens Eilers
    Pages 121-148
  6. Combining Uncaging Techniques with Patch-Clamp Recording and Optical Physiology

    • Dmitry V. Sarkisov, Samuel S.-H. Wang
    Pages 149-168
  7. Visually Guided Patch-Clamp Recordings in Brain Slices

    • James R. Moyer Jr., Thomas H. Brown
    Pages 169-227
  8. In Vivo Patch-Clamp Technique

    • Hidemasa Furue, Toshihiko Katafuchi, Megumu Yoshimura
    Pages 229-251
  9. Perforated Patch-Clamp Techniques

    • Constantine Sarantopoulos
    Pages 253-293
  10. Fast Drug Application

    • Manfred Heckmann, Stefan Hallermann
    Pages 295-307
  11. Pipette Internal Perfusion: Methods and Applications

    • Srinivas M. Tipparaju, Aruni Bhatnagar
    Pages 309-324
  12. Loose-Patch-Clamp Method

    • Héctor G. Marrero, José R. Lemos
    Pages 325-352
  13. Recording Currents from Channels and Transporters in Macropatches

    • Guiying Cui, Matthew D. Fuller, Christopher H. Thompson, Zhi-Ren Zhang, Nael A. McCarty
    Pages 353-371
  14. Planar Patch Clamping

    • Jan C. Behrends, Niels Fertig
    Pages 411-433
  15. Automated Glass Pipette-Based Patch-Clamp Techniques

    • Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter, Albrecht Lepple-Wienhues
    Pages 435-450
  16. Back Matter

    Pages 451-475

About this book

Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of research in the ever-expanding study of the cell membrane. With the latest developments in the “traditional” patch techniques such as whole-cell and single channel as well as perforated patch, fast drug application, loose patch and macropatch techniques, this authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field.

Thorough and timely, Patch-Clamp Analysis: Advanced Techniques, Second Edition is an invaluable resource for neuroscientists attempting to discover and understand the long-hidden complexities of the cell.

Reviews

From the reviews of the second edition:

"This book, edited by an outstanding expert in biophysics, and written by a large number of well-recognized contributors, is a complete text on this most important technique. … this excellent neuroscience book brings discussion of this most important technique up-to-date and discusses the current and future prospects in this field. … Patch clamp testing is becoming increasingly important in medical research. For this reason, this book can be highly recommended to medical-biological investigators." (Julian L. Ambrus and Clara M. Ambrus, The Journal of Medicine, February, 2008)

Editors and Affiliations

  • Department of Physiology, University of Saskatchewan, Saskatoon, Canada

    Wolfgang Walz

Bibliographic Information

  • Book Title: Patch-Clamp Analysis

  • Book Subtitle: Advanced Techniques

  • Editors: Wolfgang Walz

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-59745-492-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-705-1Published: 02 August 2007

  • Softcover ISBN: 978-1-61737-728-0Published: 09 November 2010

  • eBook ISBN: 978-1-59745-492-6Published: 15 March 2008

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 2

  • Number of Pages: X, 475

  • Number of Illustrations: 97 b/w illustrations

  • Topics: Neurosciences, Neurology, Human Physiology

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access