Overview
- Thorough and state-of-the-art update of the successful first edition
- Emphasis placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems
- Targeting protocols from animal, plant, fungal, and protist systems
- Chapters written by world leaders in the field
Part of the book series: Methods in Molecular Biology (MIMB, volume 390)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (30 protocols)
-
Front Matter
-
Bacterial Systems
-
Front Matter
-
-
Eukaryotic Systems
-
Front Matter
-
Keywords
About this book
In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.
Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.
Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.
Editors and Affiliations
Bibliographic Information
Book Title: Protein Targeting Protocols
Editors: Mark Giezen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-466-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-702-0Published: 21 May 2007
Softcover ISBN: 978-1-61737-727-3Published: 19 November 2010
eBook ISBN: 978-1-59745-466-7Published: 01 April 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XV, 501
Topics: Protein Science, Cell Biology, Human Genetics