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Immunological Tolerance

Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Paul J. Fairchild

    1. Sir William Dunn School of Pathology, University of Oxford, Oxford, UK

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  • Provides up-to-date reviews of the literature on essential topics in immunological tolerance
  • Documents methods for the generation and culture of critical cell types and approaches to their characterization
  • Describes protocols for the study of tolerance both in vitro and in vivo
  • Provides insights into novel techniques for tolerance induction

Part of the book series: Methods in Molecular Biology (MIMB, volume 380)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cell Types Contributing to Immunological Tolerance

    1. Front Matter

      Pages N1-N1
      Download chapter PDF

    2. Frontiers of Immunological Tolerance

      • Giorgio Raimondi, Hēeth R. Turnquist, Angus W. Thomson
      Pages 1-24

    3. Balancing Tolerance and Immunity

      • Elena Shklovskaya, Barbara Fazekas de St. Groth
      Pages 25-46

    4. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow

      • Ludovica Bruno
      Pages 47-57

    5. Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells

      • Paul J. Fairchild, Kathleen F. Nolan, Herman Waldmann
      Pages 59-72

    6. Generation of Immunocompetent T Cells from Embryonic Stem Cells

      • Renée F. de Pooter, Juan Carlos Zúñiga-Pflücker
      Pages 73-81

    7. Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells

      • Silvia Gregori, Rosa Bacchetta, Laura Passerini, Megan K. Levings, Maria Grazia Roncarolo
      Pages 83-105

    8. Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines

      • Michiyuki Kasai, Toshiaki Mizuochi
      Pages 107-123

  3. The Study of Immunological Tolerance In Vitro

    1. Front Matter

      Pages N2-N2
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    2. Thymus Organogenesis and Development of the Thymic Stroma

      • Craig S. Nowell, Alison M. Farley, C. Clare Blackburn
      Pages 125-162

    3. Generation of a Tissue-Engineered Thymic Organoid

      • Fabrizio Vianello, Mark C. Poznansky
      Pages 163-170

    4. Studying T-Cell Repertoire Selection Using Fetal Thymus Organ Culture

      • Philip G. Ashton-Rickardt
      Pages 171-184

    5. Investigating Central Tolerance With Reaggregate Thymus Organ Cultures

      • Graham Anderson, Eric J. Jenkinson
      Pages 185-196

    6. Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles

      • Marie-Lise Dion, Rafick-Pierre Sékaly, Rémi Cheynier
      Pages 197-213

    7. Gene Expression Profiling of Dendritic Cells by Microarray

      • Maria Foti, Paola Ricciardi-Castagnoli, Francesca Granucci
      Pages 215-224

    8. SAGE Analysis of Cell Types Involved in Tolerance Induction

      • Kathleen F. Nolan, Stephen P. Cobbold, Herman Waldmann
      Pages 225-251

    9. Analyzing the Physicodynamics of Immune Cells in a Three-Dimensional Collagen Matrix

      • Peter Reichardt, Frank Gunzer, Matthias Gunzer
      Pages 253-269

  4. The Study of Immunological Tolerance In Vivo

    1. Front Matter

      Pages N3-N3
      Download chapter PDF

    2. Etiology of Autoimmmune Disease

      • Eli Sercarz, Claudia Raja-Gabaglia
      Pages 271-283
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Keywords

About this book

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

Reviews

From the reviews:

"Immunological Tolerance: Methods and Protocols unites the most recent ideas and techniques of outstanding scientists in this area. … I would recommended this book, not only as a way of getting into the field of immunological tolerance, but also as a laboratory resource for the techniques required. It provides a thorough guide to the field of immunological tolerance that will be helpful to both new and established scientists wishing to investigate this exciting field." (Carl Goodyear, Immunology News, February, 2008)

"This guide provides clear information on modern laboratory techniques that are appropriate for the subject at hand -- immunological tolerance. In addition there are reviews of the literature on tolerance. … The audience for this book includes both basic science and clinical researchers who are studying problems relating to immunological tolerance. Experts in the field write the various chapters. … Thus, for anyone interested in studying tolerance, this is a potentially useful source of information." (Marion C. Cohen, Doody’s Review Service, September, 2007)

Editors and Affiliations

  • Sir William Dunn School of Pathology, University of Oxford, Oxford, UK

    Paul J. Fairchild

Bibliographic Information

  • Book Title: Immunological Tolerance

  • Book Subtitle: Methods and Protocols

  • Editors: Paul J. Fairchild

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-395-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-652-8Published: 08 June 2007

  • Softcover ISBN: 978-1-61737-696-2Published: 19 November 2010

  • eBook ISBN: 978-1-59745-395-0Published: 14 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 478

  • Number of Illustrations: 61 b/w illustrations, 5 illustrations in colour

  • Topics: Immunology, Infectious Diseases

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