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  • © 2007

Pyrosequencing Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 373)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii
  2. The History of Pyrosequencing®

    • PÃ¥l Nyrén
    Pages 1-13
  3. Pyrosequencing® Applications

    • Sharon Marsh
    Pages 15-23
  4. Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®

    • Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos et al.
    Pages 25-38
  5. Pyrosequencing®

    • Cristi R. King, Tiffany Scott-Horton
    Pages 39-55
  6. Universal Primer Applications for Pyrosequencing®

    • Dong-Chuan Guo, Dianna M. Milewicz
    Pages 57-62
  7. Allele Quantification and DNA Pooling Methods

    • Jonathon Wasson
    Pages 63-74
  8. Multiplex Pyrosequencing® for DNA Variation Analysis

    • Pritesh Patel, Yih-Horng Shiao, Paolo Fortina
    Pages 75-87
  9. Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria

    • Neil Woodford, Sarah E. North, Matthew J. Ellington
    Pages 103-114
  10. Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci

    • Steven Ringquist, Alexis Styche, William A. Rudert, Massimo Trucco
    Pages 115-133
  11. Gene Copy Number Detection in Animal Studies

    • Gerli Pielberg, Leif Andersson
    Pages 147-155
  12. Pyrosequencing® Genotype Storage Techniques

    • Derek J. Van Booven
    Pages 177-186
  13. Back Matter

    Pages 187-196

About this book

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

Editors and Affiliations

  • Division of Oncology, Washington University School of Medicine, St. Louis

    Sharon Marsh

Bibliographic Information

  • Book Title: Pyrosequencing Protocols

  • Editors: John M. Walker, Sharon Marsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597453773

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-645-0Published: 21 November 2006

  • Softcover ISBN: 978-1-61737-693-1Published: 01 December 2010

  • eBook ISBN: 978-1-59745-377-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 196

  • Topics: Biochemistry, general, Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access