Overview
- Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
- Whole genome amplification to create starting material from single cells
- Microarrays for analyzing multiple gene sequences simultaneously from a single cell
- Non-invasive assessment via polymerase chain reaction and in situ hybridization
Part of the book series: Methods in Molecular Medicine (MIMM, volume 132)
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Table of contents(13 protocols)
About this book
Reviews
From the reviews:
"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. … For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. … This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)
"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. … The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. … The chapters are written by experts and the book is carefully edited. … The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)
Editors and Affiliations
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The London Bridge Fertility, Gynecology and Genetics Centre, London, UK
Alan Thornhill
Bibliographic Information
Book Title: Single Cell Diagnostics
Book Subtitle: Methods and Protocols
Editors: Alan Thornhill
Series Title: Methods in Molecular Medicine
DOI: https://doi.org/10.1007/978-1-59745-298-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-578-1
Softcover ISBN: 978-1-61737-654-2
eBook ISBN: 978-1-59745-298-4
Series ISSN: 1543-1894
Series E-ISSN: 1940-6037
Edition Number: 1
Number of Pages: XI, 182
Topics: Biochemistry, general, Human Genetics, Molecular Medicine, Cell Biology