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  • Book
  • © 2007

Electron Microscopy

Methods and Protocols

Editors:

  • Essential electron microscopy techniques for TEM and SEM
  • Cutting-edge cryo-EM and 3D TEM techniques
  • Quantitative aspects of immunogold labeling in resin embedded samples
  • Examines the application of electron energy-loss spectroscopy (EELS) to biomedical tissue
  • Notes section enables efficient adaptation and troubleshooting of protocols
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 369)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
  2. Processing Biological Tissues for Ultrastructural Study

    • José A. Mascorro, John J. Bozzola
    Pages 19-34
  3. Recent Advances in High-Pressure Freezing

    • Kent L. McDonald, Mary Morphew, Paul Verkade, Thomas Müller-Reichert
    Pages 143-173
  4. Cryoultramicrotomy

    • Dimitri Vanhecke, Luca Studer, Daniel Studer
    Pages 175-197
  5. Cell-Free Extract Systems and the Cytoskeleton

    • Margaret Coughlin, William M. Brieher, Ryoma Ohi
    Pages 199-212
  6. Electron Microscopy In Situ Hybridization

    • DuÅ¡an Cmarko, Karel Koberna
    Pages 213-228
  7. Fluorescence-Integrated Transmission Electron Microscopy Images

    • Paul A. Sims, Jeff D. Hardin
    Pages 291-308
  8. Quantitative Immunoelectron Microscopy

    • Terry M. Mayhew
    Pages 309-329
  9. Electron Crystallography of Membrane Proteins

    • Hui-Ting Chou, James E. Evans, Henning Stahlberg
    Pages 331-343
  10. Cryoelectron Microscopy of Icosahedral Virus Particles

    • Wen Jiang, Wah Chiu
    Pages 345-363
  11. Whole-Mount Immunoelectron Tomography of Chromosomes and Cells

    • Peter Engelhardt, Jari Meriläinen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui, Veli-Pekka Lehto
    Pages 387-405

About this book

In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Reviews

"This is an excellent book, with well-written chapters.  The protocols are easy to follow and well organized.  This book is highly recommended for beginners to advanced level microscopists." -Micron

Editors and Affiliations

  • Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Australia

    John Kuo

Bibliographic Information

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access