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Human Embryonic Stem Cell Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Health Research Institute, Ottawa, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 331)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Human Embryonic Stem Cells

    • Kursad Turksen, Tammy-Claire Troy
    Pages 1-12

  3. Blastocyst Culture for Deriving Human Embryonic Stem Cells

    • Ariff Bongso
    Pages 13-22

  4. Propagation of Human Embryonic Stem Cells on Human Feeder Cells

    • Mark Richards, Ariff Bongso
    Pages 23-41

  5. Derivation and Maintenance of Human Embryonic Stem Cells

    • Michal Amit, Joseph Itskovitz-Eldor
    Pages 43-53

  6. Isolation and Characterization of Human Embryonic Stem Cells

    • Maisam Mitalipova, Gail Palmarini
    Pages 55-76

  7. Routine Culture and Differentiation of Human Embryonic Stem Cells

    • Jim McWhir, Davina Wojtacha, Alison Thomson
    Pages 77-90

  8. Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells

    • Gautam Dravid, Holly Hammond, Linzhao Cheng
    Pages 91-104

  9. Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions

    • Michal Amit, Joseph Itskovitz-Eldor
    Pages 105-113

  10. Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inhibitor

    • Noboru Sato, Ali H. Brivanlou
    Pages 115-128

  11. Derivation of Human Feeders for Prolonged Support of Human Embryonic Stem Cells

    • Chui-Yee Fong, Ariff Bongso
    Pages 129-135

  12. Transplantation of Human Embryonic Stem Cells to the Chick Embryo

    • Ronald S. Goldstein
    Pages 137-152

  13. Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic Stem Cells In Vitro

    • Melissa Carpenter, Mahendra S. Rao, William J. Freed, Xianmin Zeng
    Pages 153-167

  14. In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells

    • Xue-Jun Li, Su-Chun Zhang
    Pages 169-177

  15. Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells

    • Lisheng Wang, Chantal Cerdan, Pablo Menendez, Mickie Bhatia
    Pages 179-200

  16. Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells

    • Pablo Menendez, Lisheng Wang, Chantal Cerdan, Mickie Bhatia
    Pages 201-220

  17. Genetic Manipulation of Human Embryonic Stem Cells by Transfection

    • Rachel Eiges
    Pages 221-239

  18. Designing, Testing, and Validating a Microarray for Stem Cell Characterization

    • Yongquan Luo, Bhaskar Bhattacharya, Amy X. Yang, Raj K. Puri, Mahendra S. Rao
    Pages 241-266

  19. Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells

    • Noboru Sato, Ali H. Brivanlou
    Pages 267-283

  20. Massively Parallel Signature Sequencing

    • Daixing Zhou, Mahendra S. Rao, Roger Walker, Irina Khrebtukova, Christian D. Haudenschild, Takumi Miura et al.
    Pages 285-311
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Keywords

About this book

A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

"The book presents 22 chapters from an international group of experts in the field … . Human Embryonic Stem Cell Protocols is designed to provide a collection of the essential methods in hESC culture and maintenance … . a comprehensive set of valuable protocols from leading research labs in the world. … This book is a valuable resource for hESC investigators who hope to expand their understanding of the manipulation of hESCs." (Elayne Chan and George Q. Daley, Bone Marrow Transplantation, May, 2006)

Editors and Affiliations

  • Ottawa Health Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Human Embryonic Stem Cell Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597450464

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • eBook ISBN: 978-1-59745-046-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 386

  • Topics: Cell Biology, Cell Culture

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