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  • © 2008

Apoptosis and Cancer

Methods and Protocols

  • Readily reproducible, cutting-edge techniques for studying the biology of apoptosis
  • Step-by-step instructions that ensure successful results
  • Tricks of the trade and notes on troubleshooting and avoiding known pitfalls

Part of the book series: Methods in Molecular Biology (MIMB, volume 414)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Modulation of Apoptosis to Reverse Chemoresistance

    • Gil Mor, Michele K. Montagna, Ayesha B. Alvero
    Pages 1-12
  3. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis

    • Suparna Mazumder, Dragos Plesca, Alexandru Almasan
    Pages 13-21
  4. Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate

    • David Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Jorge Monserrat, Melchor Alvarez-Mon
    Pages 23-33
  5. Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection

    • Dan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna et al.
    Pages 35-45
  6. Flow Cytometric Detection of Activated Caspase-3

    • Richard Fox, Martine Aubert
    Pages 47-56
  7. Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays

    • Elah Pick, Mary M. McCarthy, Harriet M. Kluger
    Pages 83-93
  8. Mitochondria Potential, Bax “Activation,” and Programmed Cell Death

    • C. Michael Knudson, Nicholas M. Brown
    Pages 95-108
  9. In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant Fluorescent-Labeled Inhibitors of Caspases

    • Brian W. Lee, Michael R. Olin, Gary L. Johnson, Robert J. Griffin
    Pages 109-135
  10. Caspase Activity Assays

    • Andrew L. Niles, Richard A. Moravec, Terry L. Riss
    Pages 137-150
  11. Multiplex Caspase Activity and Cytotoxicity Assays

    • Andrew L. Niles, Richard A. Moravec, Terry L. Riss
    Pages 151-162
  12. Homogeneous, Bioluminescent Proteasome Assays

    • Martha A. O′Brien, Richard A. Moravec, Terry L. Riss, Robert F. Bulleit
    Pages 163-181
  13. A Simple Method for Profiling miRNA Expression

    • Jia-Wang Wang, Jin Q. Cheng
    Pages 183-190
  14. Apoptotic Caspase Activation and Activity

    • Jean-Bernard Denault, Guy S. Salvesen
    Pages 191-220
  15. Back Matter

    Pages 253-266

About this book

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors – all leaders in the field – have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis.

Reviews

From the reviews:

"Divided among 17 chapters, the condensed information about apoptosis, its pathways and the performance of progressive techniques for apoptosis assessment are presented. … The valuable and most appreciated part of each chapter are notes – the reader will find here the plethora of hints, the real grain of wisdom, virtual wizard that saves a lot of struggling time at benchtop. This book belongs to the category of must-have books." (J. Sedlák, Neoplasma, September, 2008)

Editors and Affiliations

  • Department of Obstetrics Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven

    Gil Mor, Ayesha B. Alvero

Bibliographic Information

  • Book Title: Apoptosis and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Gil Mor, Ayesha B. Alvero

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-339-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-457-9Published: 11 October 2007

  • Softcover ISBN: 978-1-61737-582-8Published: 19 November 2010

  • eBook ISBN: 978-1-59745-339-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 266

  • Number of Illustrations: 69 b/w illustrations

  • Topics: Oncology, Cancer Research, Cell Biology, Molecular Medicine, Biochemistry, general

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access