Overview
- Editors:
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Paul J. Verma
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Centre for Reproduction and Development Monash Institute of Medical Research, Monash University, Melbourne, Australia
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Alan O. Trounson
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Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Australia
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Table of contents (22 protocols)
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Nuclear Transfer
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Front Matter
Pages 197-197
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Culture and Maintenance of Nuclear Transfer Embryos
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- Marc-André Sirard, Karine Coenen
Pages 35-42
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- Björn Heindryckx, Josiane Van der Elst, Marc Dhont
Pages 59-77
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- Tim King, Paul A. De Sousa
Pages 79-90
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- Jacqui T. Forsyth, David N. Wells
Pages 91-108
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Nuclear Transfer Methods
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- Young Gie Chung, Shaorong Gao, Keith E. Latham
Pages 111-123
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- Kazuchika Miyoshi, Sezen Arat, Steven L. Stice
Pages 125-134
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- Irina A. Polejaeva, Shawn Walker, Keith Campbell
Pages 135-149
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- Shoukhrat M. Mitalipov, Don P. Wolf
Pages 151-168
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- James “Buck” Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon et al.
Pages 169-181
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- Gábor Vajta, Ian M. Lewis, R. Tayfur Tecirlioglu
Pages 183-195
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Trangenesis
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- A. John Clark, Sarah Burl, Chris Denning
Pages 199-212
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- Athoula Lazaris, Rebecca Keyston, Costas N. Karatzas, Carol L. Keefer
Pages 213-226
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Reprogramming Nuclei
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Front Matter
Pages 227-227
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- Takashi Kohda, Fumitoshi Ishino, Atsuo Ogura
Pages 237-246
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- Koichi Gonda, Nobuaki Kikyo
Pages 247-258
About this book
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Reviews
"...an essential comprehensive handbook for both research and commercials labs involved in nuclear transfer, and will be of interest to molecular and stem cell biologists and biotechnologists involved with reprogramming, nuclear transfer and transgensis." -Biotech-online
Editors and Affiliations
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Centre for Reproduction and Development Monash Institute of Medical Research, Monash University, Melbourne, Australia
Paul J. Verma
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Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Australia
Alan O. Trounson
