NanoBiotechnology Protocols

1. Front Matter

   Pages i-xii

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2. Applications of Quantum Dots in Biology

   • Charles Z. Hotz

   Pages 1-17

3. Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots

   • Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, J. Matthew Mauro

   Pages 19-34

4. Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates

   • John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, Randy D. Blakely

   Pages 35-50

5. Peptide-Conjugated Quantum Dots

   • Ian D. Tomlinson, John N. Mason, Randy D. Blakely, Sandra J. Rosenthal

   Pages 51-60

6. Quantum Dot-Encoded Beads

   • Xiaohu Gao, Shuming Nie

   Pages 61-71

7. Use of Nanobarcodes® Particles in Bioassays

   • R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He et al.

   Pages 73-83

8. Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging

   • Alexander Tkachenko, Huan Xie, Stefan Franzen, Daniel L. Feldheim

   Pages 85-99

9. Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies

   • Lee R. Hirsch, Naomi J. Halas, Jennifer L. West

   Pages 101-111

10. Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters

    • Jennifer Edl, Ray Mernaugh, David W. Wright

    Pages 113-120

11. Surface-Functionalized Nanoparticles for Controlled Drug Delivery

    • Sung-Wook Choi, Woo-Sik Kim, Jung-Hyun Kim

    Pages 121-131

12. Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis

    • Joseph M. Slocik, David W. Wright

    Pages 133-141

13. Structural DNA Nanotechnology

    • Nadrian C. Seeman

    Pages 143-166

14. Nanostructured DNA Templates

    • Jeffery L. Coffer, Russell F. Pinizzotto, Young Gyu Rho

    Pages 167-178

15. Probing DNA Structure With Nanoparticles

    • Rahina Mahtab, Catherine J. Murphy

    Pages 179-190

16. Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells

    • Timothy E. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, Michael L. Simpson

    Pages 191-208

17. Real-Time Cell Dynamics With a Multianalyte Physiometer

    • Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, David E. Cliffel

    Pages 209-223

18. Back Matter

    Pages 224-228

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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.

• DNA
• biotechnology
• cellular processes
• drug discovery
• protein

• The Department of Chemistry, Vanderbilt University, Nashville

  Sandra J. Rosenthal, David W. Wright

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