Vaccinia Virus and Poxvirology

1. Front Matter

   Pages i-xix

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2. Working Safely with Vaccinia Virus

   • Stuart N. Isaacs

   Pages 1-13

3. Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers

   • Maríia M. Lorenzo, Inmaculada Galindo, Rafael Blasco

   Pages 15-30

4. Construction of Recombinant Vaccinia Virus

   • Chelsea M. Byrd, Dennis E. Hruby

   Pages 31-40

5. Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses

   • Mohamed Ragaa Mohamed, Edward G. Niles

   Pages 41-50

6. Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA

   • Xiao-Dan Yao, David H. Evans

   Pages 51-64

7. Construction of cDNA Libraries in Vaccinia Virus

   • Ernest S. Smith, Shuying Shi, Maurice Zauderer

   Pages 65-75

8. Construction and Isolation of Recombinant MVA

   • Caroline Staib, Ingo Drexler, Gerd Sutter

   Pages 77-99

9. Growing Poxviruses and Determining Virus Titer

   • Girish J. Kotwal, Melissa-Rose Abrahams

   Pages 101-112

10. Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR

    • Rachel L. Roper

    Pages 113-118

11. Orthopoxvirus Diagnostics

    • Hermann Meyer, Inger K. Damon, Joseph J. Esposito

    Pages 119-133

12. An In Vitro Transcription System for Studying Vaccinia Virus Early Genes

    • Steven S. Broyles, Marcia Kremer

    Pages 135-142

13. An In Vitro Transcription System for Studying Vaccinia Virus Late Genes

    • Cynthia F. Wright

    Pages 143-150

14. Studying Vaccinia Virus RNA Processing In Vitro

    • Paul D. Gershon

    Pages 151-167

15. Methods for Analysis of Poxvirus DNA Replication

    • Paula Traktman, Kathleen Boyle

    Pages 169-185

16. Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus

    • Mansun Law, Geoffrey L. Smith

    Pages 187-203

17. Pox, Dyes, and Videotape

    • Brian M. Ward

    Pages 205-218

18. Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance

    • Bruce T. Seet, Grant McFadden

    Pages 219-242

19. Monitoring of Human Immunological Responses to Vaccinia Virus

    • Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, Miles W. Carroll

    Pages 243-265

20. Vaccinia Virus as a Tool for Immunologic Studies

    • Nia Tatsis, Gomathinayagam Sinnathamby, Laurence C. Eisenlohr

    Pages 267-287

The Right Book at the Right Time The poxviruses comprise a family of complex DNA viruses that replicate in the cytoplasm of vertebrate or invertebrate cells. Of the eight recognized g- era of vertebrate poxviruses, those belonging to the orthopoxvirus genus have been most intensively studied. This group includes variola virus, the agent of smallpox, as well as cowpox virus and vaccinia virus. Jenner’s original sma- pox vaccine, described in 1798, consisted of live cowpox virus, but vaccinia virus later replaced it (1). There has been speculation as to the origin of v- cinia virus; the most likely idea is that it is a separate species, possibly ori- nally isolated from a horse, and is now extinct or rare in nature (2). Recent genome sequencing studies confirm the distinctness of variola virus, cowpox virus, and vaccinia virus and also their very close genetic relationship, which accounts for the cross protection of smallpox vaccines. The novelty of the smallpox vaccine can be readily appreciated by the time it took, about 80 years, before the next live vaccine against rabies was developed, and another 50 years for the yellow fever vaccine. Moreover, the eradication of smallpox in 1977 stands as a unique medical achievement. Because of its historical role, sma- pox vaccination contributed greatly to present concepts of infectious disease, immunity, and pathogenesis. Less well known, however, are the many other “firsts” for vaccinia virus.

From the foreword...

"The right book at the right time." -Bernard Moss

"...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapy

• Division of Infectious Diseases, University of Pennsylvania, Philadelphia

  Stuart N. Isaacs

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