Overview
- Editors:
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Angelo Corti
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Department of Biological and Technological Research, Cancer Immunotherapy and Gene Therapy Program, San Raffaele H Scientific Institute, Milan, Italy
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Pietro Ghezzi
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Mario Negri Institute for Pharmacological Research, Milan, Italy
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Table of contents (18 protocols)
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- Pietro Ghezzi, Anthony Cerami
Pages 1-8
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- Flavio Curnis, Angelo Corti
Pages 9-22
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- Hartmut Engelmann, Dani Aderka, David Wallach
Pages 23-31
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- Paul Ameloot, Peter Brouckaert
Pages 33-45
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- Silvia Fargion, Luca Valenti, Paola Dongiovanni, Anna Ludovica Fracanzani
Pages 47-58
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- Roberto Furlan, Giuliana Salani, Alessandra Bergami, Gianvito Martino
Pages 59-72
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- Paolo Bigini, Tiziana Mennini
Pages 73-79
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- Marta Muzio, Simona Saccani
Pages 81-99
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- Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D’Herde, Peter Vandenabeele
Pages 101-126
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- Eleni Douni, Maria Alexiou, George Kollias
Pages 137-169
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- Roberto Furlan, Pia Villa, Giorgio Senaldi, Gianvito Martino
Pages 171-190
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- Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, Roberto Latini
Pages 217-225
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- Timo L. M. ten Hagen, Alexander M. M. Eggermont
Pages 227-245
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Back Matter
Pages 265-270
About this book
Because tumor necrosis factor-a (TNF-a) plays a pivotal role in the regulation of homeostasis and inflammatory immune responses, it offers valuable research opportunities to develop new drugs for the treatment of a wide range of disorders, including cancer, septic shock, rheumatoid arthritis, and other inflammatory diseases. In Tumor Necrosis Factor: Methods and Protocols, well-versed experimentalists survey the basic and translational research being conducted in this field and describe in detail the methods they have developed for TNF production, characterization, mutagenesis, and detection in biological specimens. They also provide several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The protocols presented follow the successful Methods in Molecular Medicineâ„¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Tumor Necrosis Factor: Methods and Protocols offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of productive, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.
Reviews
"...offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of productive, cutting-edge tools for illuminating pathophysiological roles of TNF in disease and for identifying new drugs." - Tumori
Editors and Affiliations
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Department of Biological and Technological Research, Cancer Immunotherapy and Gene Therapy Program, San Raffaele H Scientific Institute, Milan, Italy
Angelo Corti
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Mario Negri Institute for Pharmacological Research, Milan, Italy
Pietro Ghezzi
