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Human Cell Culture Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Joanna Picot

    1. Southampton, UK

    View editor publications

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 107)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Establishment and Maintenance of Normal Human Keratinocyte Cultures

    • Claire Linge
    Pages 1-11

  3. Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters

    • Mei-Yu Hsu, Ling Li, Meenhard Herlyn
    Pages 13-28

  4. Isolation and Culture of Human Osteoblasts

    • Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, James A. Gallagher
    Pages 29-54

  5. Human Osteoclast Culture From Peripheral Blood Monocytes

    • Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, James A. Gallagher
    Pages 55-68

  6. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation

    • Mary B.
    Pages 69-95

  7. Human Myoblasts and Muscle-Derived SP Cells

    • Grace K. Pavlath, Emanuela Gussoni
    Pages 97-110

  8. Cell Cultures of Autopsy-Derived Fibroblasts

    • Volker Meske, Frank Albert, Thomas G. Ohm
    Pages 111-123

  9. Primary Culture and Differentiation of Human Adipocyte Precursor Cells

    • Vanessa van Harmelen, Thomas Skurk, Hans Hauner
    Pages 125-135

  10. Human Mononuclear Phagocytes in Tissue Culture

    • Yona Keisari
    Pages 137-145

  11. Purification of Peripheral Blood Natural Killer Cells

    • Bice Perussia, Matthew J. Loza
    Pages 147-161

  12. Human Fetal Brain Cell Culture

    • Mark P. Mattson
    Pages 163-171

  13. Culturing Human Schwann Cells

    • Victor J. Turnbull
    Pages 173-182

  14. Well-Differentiated Human Airway Epithelial Cell Cultures

    • M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, Scott H. Randell
    Pages 183-206

  15. Isolation and Culture of Human Alveolar Epithelial Cells

    • Carsten Ehrhardt, Kwang-Jin Kim, Claus-Michael Lehr
    Pages 207-216

  16. A New Approach to Primary Culture of Human Gastric Epithelium

    • Pierre Chailler, Daniel Ménard
    Pages 217-236

  17. Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach

    • Hamid A. Mohammadpour
    Pages 237-247

  18. Isolation and Culture of Human Hepatocytes

    • Martin Bayliss, Graham Somers
    Pages 249-267

  19. Glomerular Epithelial and Mesangial Cell Culture and Characterization

    • Heather M. Wilson, Keith N. Stewart
    Pages 269-282

  20. Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules

    • Gabrielle M. Hawksworth
    Pages 283-290
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Keywords

About this book

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Reviews

"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function

"...a very useful general text for researchers involved in human cell cultures. -Immunology News

From Reviews of the First Edition...

"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology

"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology

"...will be of significance to a very wide range of scientists." -ISSX Newsletter

"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology

"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter

 

Editors and Affiliations

  • Southampton, UK

    Joanna Picot

Bibliographic Information

  • Book Title: Human Cell Culture Protocols

  • Editors: Joanna Picot

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598617

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-222-3Published: 15 October 2004

  • Softcover ISBN: 978-1-61737-431-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-861-8Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 2

  • Number of Pages: XII, 360

  • Number of Illustrations: 120 b/w illustrations

  • Topics: Cell Biology

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