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  • Book
  • © 2004

Checkpoint Controls and Cancer

Volume 1: Reviews and Model Systems

Editors:

  1. Axel H. Schönthal

    1. Department of Molecular Microbiology and Immunology and K. Norris Jr. Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles

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Part of the book series: Methods in Molecular Biology (MIMB, volume 280)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Reviews of Checkpoint Controls, Their Involvement in the Development of Cancer, and Approaches to Their Investigation

    1. Front Matter

      Pages 1-1
      PDF

    3. Analyzing the G2/M Checkpoint

      • George R. Stark, William R. Taylor
      Pages 51-82

    4. Analyzing the Spindle Checkpoint in Yeast and Frogs

      • P. Todd Stukenberg, Daniel J. Burke
      Pages 83-98

    5. Cell Cycle Checkpoint Control Mechanisms That Can Be Disrupted in Cancer

      • Bipin C. Dash, Wafik S. El-Deiry
      Pages 99-161

  3. Analyzing Checkpoint Controls in Diverse Model Systems

    1. Front Matter

      Pages 163-163
      PDF

    3. Analyzing Checkpoint Controls in Human Skin

      • Sandra Pavey, Brian G. Gabrielli
      Pages 175-183

    4. Generation and Analysis of Brca1 Conditional Knockout Mice

      • Chu-Xia Deng, Xiaoling Xu
      Pages 185-200

    6. Xenopus Cell-Free Extracts to Study the DNA Damage Response

      • Vincenzo Costanzo, Kirsten Robertson, Jean Gautier
      Pages 213-227

    8. Control of Mitotic Entry After DNA Damage in Drosophila

      • Burnley Jaklevic, Amanda Purdy, Tin Tin Su
      Pages 245-256

    9. Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans

      • Anton Gartner, Amy J. MacQueen, Anne M. Villeneuve
      Pages 257-274

    10. Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae

      • Christopher M. Yellman, Daniel J. Burke
      Pages 275-290

  4. Back Matter

    Pages 307-318
    PDF
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About this book

Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced—for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer.
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Editors and Affiliations

  • Department of Molecular Microbiology and Immunology and K. Norris Jr. Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles

    Axel H. Schönthal

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Bibliographic Information

  • Book Title: Checkpoint Controls and Cancer

  • Book Subtitle: Volume 1: Reviews and Model Systems

  • Editors: Axel H. Schönthal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597882

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-214-8Published: 04 June 2004

  • Softcover ISBN: 978-1-61737-426-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-788-8Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 318

  • Topics: Cancer Research

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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