Cell Imaging Techniques

1. Front Matter

   Pages i-xiv

   PDF

2. Molecular Beacons

   • Diana P. Bratu

   Pages 1-14

3. Second-Harmonic Imaging of Collagen

   • Guy Cox, Eleanor Kable

   Pages 15-35

4. Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy

   • Michael Wm. Roe, Jerome F. Fiekers, Louis H. Philipson, Vytautas P. Bindokas

   Pages 37-66

5. Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue

   • Maria Stern, Douglas J. Taatjes, Brooke T. Mossman

   Pages 67-76

6. Evaluation of Confocal Microscopy System Performance

   • Robert M. Zucker

   Pages 77-135

7. Quantitative Analysis of Atherosclerotic Lesion Composition in Mice

   • Marilyn P. Wadsworth, Burton E. Sobel, David J. Schneider, Wendy Tra, Hans van Hirtum, Douglas J. Taatjes

   Pages 137-152

8. Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases

   • Thomas O. Moninger, Randy A. Nessler, Kenneth C. Moore

   Pages 153-163

9. Laser Scanning Cytometry

   • Piotr Pozarowski, Elena Holden, Zbigniew Darzynkiewicz

   Pages 165-192

10. Near-Clinical Applications of Laser Scanning Cytometry

    • David A. Rew, Gerrit Woltmann, Davinder Kaur

    Pages 193-212

11. Laser Capture Microdissection

    • Virginia Espina, John Milia, Glendon Wu, Stacy Cowherd, Lance A. Liotta

    Pages 213-229

12. Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction

    • Christopher B. Manning, Brooke T. Mossman, Douglas J. Taatjes

    Pages 231-236

13. New Approaches to Fluorescence In Situ Hybridization

    • Sabita K. Murthy, Douglas J. Demetrick

    Pages 237-259

14. Microarray Image Scanning

    • Latha Ramdas, Wei Zhang

    Pages 261-273

15. Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics

    • Michael Hausmann, Birgit Perner, Alexander Rapp, Leo Wollweber, Harry Scherthan, Karl-Otto Greulich

    Pages 275-294

16. Porosome

    • Bhanu P. Jena

    Pages 295-316

17. Secretory Vesicle Swelling by Atomic Force Microscopy

    • Sang-Joon Cho, Bhanu P. Jena

    Pages 317-330

18. Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope

    • Kevin D. Costa

    Pages 331-361

19. Reflection Contrast Microscopy

    • F. A. Prins, I. Cornelese-ten Velde, E. de Heer

    Pages 363-401

20. Three-Dimensional Analysis of Single Particles by Electron Microscopy

    • Teresa Ruiz, Michael Radermacher

    Pages 403-425

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

• Microarray
• cell biology
• electron microscopy
• gene expression
• genes
• hybridization
• transmission electron microscopy

From the reviews:

"...deserves to do well." -Ultramicroscopy

"The book covers a wide range of experimental imaging methods for the investigation of cells. … The book is well produced, with many excellent photographs and diagrams, and I have enjoyed broadening my awareness of techniques that not only did I not know in detail but in some cases not even know existed. A nice book … ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)

"This 21-chapter volume … provides detailed descriptions of and protocols for a plethora of microscopy and related methods to study tissues, cells and macromolecules. … This comprehensive and well rounded book is suitable for institutional or personal purchase, novices and experts, and it is certainly a must-read for all serious confocal microscopists and imaging facility managers." (Andreas Holzenburg, Microbiology Today, August, 2006)

"Douglas Taatjes and Brooke Mossman have edited a compilation of 21 articles. … The subjects covered are very diverse, ranging from light microscopy to electron microscopy. … In summary, this book provides an excellent overview on current and relevant imaging techniques. The described methods and protocols are not only of particular interest to those who are new in the field or who have to expand their imaging capability above regular light microscopy. The book will serve as a reference for years to come … ." (Carsten Schultz, ChemBioChem, Vol. 7, 2006)

"This text will be extremely useful. … it is a collection of advanced and sometimes disparate microscopy techniques and their application to biological research. … The authors include highly detailed descriptions and instructions on the low-level methods computer programmers use for acquisition, storage, and manipulation of digital image data." (Steven Ruzin, Microscope, Vol. 54, 2006)

• Department of Pathology, University of Vermont, Burlington

  Douglas J. Taatjes, Brooke T. Mossman

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