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Molecular Toxicology Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Phouthone Keohavong

    1. Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

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    You can also search for this editor in PubMed   Google Scholar

  2. Stephen G. Grant

    1. Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 291)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Analysis of DNA Adducts

    1. Front Matter

      Pages 1-1
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    2. 32P-Postlabeling Analysis of DNA Adducts

      • David H. Phillips, Alan Hewer, Volker M. Arlt
      Pages 3-12

    3. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

      • Masako Ochiai, Takashi Sugimura, Minako Nagao
      Pages 13-19

    4. DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry

      • Karen H. Dingley, Esther A. Ubick, John S. Vogel, Kurt W. Haack
      Pages 21-27

    5. Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles

      • Iovanna Pandelova, Stephen R. Hewitt, John B. Hays
      Pages 29-38

    6. Analysis of DNA Strand Cleavage at Abasic Sites

      • Walter A. Deutsch, Vijay Hegde
      Pages 39-46

  3. Detection of Chromosomal and Genome-Wide Damage

    1. Front Matter

      Pages 47-47
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    2. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes

      • Pataje G. S. Prasanna, William F. Blakely
      Pages 49-57

    3. Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk

      • Xifeng Wu, Yun-Ling Zheng, T. C. Hsu
      Pages 59-67

    4. Flow Cytometric Analysis of Micronuclei in Erythrocytes

      • Jan Grawé
      Pages 69-83

    5. The Comet Assay

      • Günter Speit, Andreas Hartmann
      Pages 85-95

    6. Computerized Image Analysis Software for the Comet Assay

      • R. C. Chaubey
      Pages 97-106

    7. The Comet-FISH Technique

      • Alexander Rapp, Michael Hausmann, Karl Otto Greulich
      Pages 107-119

    8. DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis

      • Nina Joshi, Stephen G. Grant
      Pages 121-129

  4. Detection and Characterization of Surrogate Gene Mutation

    1. Front Matter

      Pages 131-131
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    2. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes

      • Vasily N. Dobrovolsky, Joseph G. Shaddock, Robert H. Heflich
      Pages 133-144

    3. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

      • Roy R. Swiger
      Pages 145-153

    4. Methods for Detecting Somatic Mutations In Vitro

      • Sai-Mei Hou
      Pages 155-160

    5. Molecular Analysis of Mutations in the Human HPRT Gene

      • Phouthone Keohavong, Liqiang Xi, Stephen G. Grant
      Pages 161-170
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Keywords

About this book

A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.

Reviews

"This book should be of interest to both specialist researchers as well as members of the wider scientific community..." - British Journal of Anesthesiology

Editors and Affiliations

  • Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

    Phouthone Keohavong, Stephen G. Grant

Bibliographic Information

  • Book Title: Molecular Toxicology Protocols

  • Editors: Phouthone Keohavong, Stephen G. Grant

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598404

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Softcover ISBN: 978-1-61737-347-3Published: 21 September 2011

  • eBook ISBN: 978-1-59259-840-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 489

  • Topics: Pharmacology/Toxicology, Biochemistry, general

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